10.5061/DRYAD.J1PT126
Panganiban, Brian
University of California, Berkeley
Qiao, Baofu
Northwestern University
Jiang, Tao
University of California, Berkeley
DelRe, Christopher
University of California, Berkeley
Obadia, Mona M.
Claude Bernard University Lyon 1
Nguyen, Trung Dac
Northwestern University
Smith, Anton A. A.
University of California, Berkeley
Hall, Aaron
University of California, Berkeley
Sit, Izaac
University of California, Berkeley
Crosby, Marquise G.
Wright-Patterson Air Force Base
Dennis, Patrick B.
Wright-Patterson Air Force Base
Drockenmuller, Eric
Claude Bernard University Lyon 1
Olvera de la Cruz, Monica
Northwestern University
Xu, Ting
University of California, Berkeley
Data from: Random heteropolymers preserve protein function in foreign
environments
Dryad
dataset
2019
2019-02-13T00:00:00Z
2019-02-13T00:00:00Z
en
https://doi.org/10.1126/science.aao0335
23088713 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The successful incorporation of active proteins into synthetic polymers
could lead to a new class of materials with functions found only in living
systems. However, proteins rarely function under the conditions suitable
for polymer processing. On the basis of an analysis of trends in protein
sequences and characteristic chemical patterns on protein surfaces, we
designed four-monomer random heteropolymers to mimic intrinsically
disordered proteins for protein solubilization and stabilization in
non-native environments. The heteropolymers, with optimized composition
and statistical monomer distribution, enable cell-free synthesis of
membrane proteins with proper protein folding for transport and
enzyme-containing plastics for toxin bioremediation. Controlling the
statistical monomer distribution in a heteropolymer, rather than the
specific monomer sequence, affords a new strategy to interface with
biological systems for protein-based biomaterials.
Sequence simulation of random heteropolymerThe file contains simulated
sequences of random heteropolymers and block length histogram
analysis.Sequence Simulation.zipSurface AnalysisSurface analysis of water
soluble proteins as a function of surface chemistryEnzyme-Polymer
SimulationSimulation of protein-random heteropolymer complex in solutions