10.5061/DRYAD.HV01F
Ouédraogo, André Lin
Centre National de Recherche et de Formation sur le Paludisme
Bousema, Teun
London School of Hygiene & Tropical Medicine
Schneider, Petra
University of Edinburgh
de Vlas, Sake J.
Erasmus University Medical Center
Ilboudo-Sanogo, Edith
Centre National de Recherche et de Formation sur le Paludisme
Cuzin-Ouattara, Nadine
Centre National de Recherche et de Formation sur le Paludisme
Nébié, Issa
Centre National de Recherche et de Formation sur le Paludisme
Roeffen, Will
Radboud University Nijmegen Medical Centre
Verhave, Jan Peter
Radboud University Nijmegen Medical Centre
Luty, Adrian J.F.
Radboud University Nijmegen Medical Centre
Sauerwein, Robert
Radboud University Nijmegen Medical Centre
Data from: Substantial contribution of submicroscopical Plasmodium
falciparum gametocyte carriage to the infectious reservoir in an area of
seasonal transmission.
Dryad
dataset
2013
Membrane feeding assay
Plasmodium falciparum
Malaria
Anopheles gambiae
2013-05-15T14:56:19Z
2013-05-15T14:56:19Z
en
https://doi.org/10.1371/journal.pone.0008410
17372 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Background: Man to mosquito transmission of malaria depends on the
presence of the sexual stage parasites, gametocytes, that often circulate
at low densities. Gametocyte densities below the microscopical threshold
of detection may be sufficient to infect mosquitoes but the importance of
submicroscopical gametocyte carriage in different transmission settings is
unknown. Methodology/Principal Findings: Membrane feeding experiments were
carried out on 80 children below 14 years of age at the end of the wet
season in an area of seasonal malaria transmission in Burkina Faso.
Gametocytes were quantified by microscopy and by Pfs25-based quantitative
nucleic acid sequence-based amplification assay (QT-NASBA). The
children's infectiousness was determined by membrane feeding
experiments in which a venous blood sample was offered to locally reared
Anopheles mosquitoes. Gametocytes were detected in 30.0% (24/80) of the
children by microscopy compared to 91.6% (65/71) by QT-NASBA
(p<0.001). We observed a strong association between QT-NASBA
gametocyte density and infection rates (p = 0.007). Children with
microscopically detectable gametocytes were more likely to be infectious
(68.2% compared to 31.7% of carriers of submicroscopical gametocytes, p =
0.001), and on average infected more mosquitoes (13.2% compared to 2.3%,
p<0.001). However, because of the high prevalence of
submicroscopical gametocyte carriage in the study population, carriers of
sub-microscopical gametocytes were responsible for 24.2% of the malaria
transmission in this population. Conclusions/Significance:
Submicroscopical gametocyte carriage is common in an area of seasonal
transmission in Burkina Faso and contributes substantially to the human
infectious reservoir. Submicroscopical gametocyte carriage should
therefore be considered when implementing interventions that aim to reduce
malaria transmission.
Characteristics of membrane feeding assay blood donors and mosquito
infectionshost = id of the host. calib_no = the calibration line number
used to convert TTP (time to positivity readouts) from QT-NASBA into
gametocyte density. ttp = time to positivity readout from QT-NASBA used to
generate gametocyte density estimate. age = age of blood donor. asexual =
asexual parasite density of blood donor (estimated using microscopy).
no_dissected = number of mosquitoes dissected to determine mosquito
infection. no_infected = number of mosquitoes dissected with observable
oocystsBF_data.xlsx
Burkina Faso