10.5061/DRYAD.H8FP2
Herrera, Carlos M.
Spanish National Research Council
Medrano, Mónica
Spanish National Research Council
Bazaga, Pilar
Spanish National Research Council
Data from: Comparative spatial genetics and epigenetics of plant
populations: heuristic value and a proof of concept
Dryad
dataset
2016
Spatial structure
isolation by distance
isolation by distance
Ranunculaceae
epigenetic diversity
DNA methylation
Spermatophyta
Helleborus foetidus
Spatial structure
Holocene
2016-02-02T18:26:37Z
2016-02-02T18:26:37Z
en
https://doi.org/10.1111/mec.13576
51521 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Despite the recent upsurge of interest on natural epigenetic variation of
nonmodel organisms, factors conditioning the spatial structure of
epigenetic diversity in wild plant populations remain virtually
unexplored. We propose that information on processes shaping natural
epigenetic variation can be gained using the spatial structure of genetic
diversity as null model. Departures of epigenetic isolation-by-distance
(IBD) patterns from genetic IBD patterns for the same sample, particularly
differences in slope of similarity-distance regressions, will reflect the
action of factors that operate specifically on epigenetic variation,
including imperfect transgenerational inheritance and responsiveness to
environmental factors of epigenetic marks. As a proof of concept, we
provide a comparative analysis of spatial genetic and epigenetic structure
of 200 mapped individuals of the perennial herb Helleborus foetidus.
Plants were fingerprinted using nuclear microsatellites, amplified
fragment length polymorphisms (AFLP) and methylation-sensitive AFLP
markers. Expectations from individual-level IBD patterns were tested by
means of kinship-distance regressions. Both genetic and epigenetic
similarity between H. foetidus individuals conformed to theoretical
expectations under individual-level IBD models. Irrespective of marker
type, there were significant negative linear relationships between the
kinship coefficient for plant pairs and their spatial separation.
Regression slopes were significantly steeper for epigenetic markers.
Epigenetic similarity between individuals was much greater than genetic
similarity at shortest distances, such epigenetic ‘kinship excess’ tending
to decrease as plant separation increased. Results suggest that
moderate-to-high heritability and responsiveness to local environments are
major drivers of epigenetic spatial structure in H. foetidus, and
illustrate the heuristic value of comparing genetic and epigenetic spatial
structure for formulating and testing hypotheses on forces shaping
epigenetic diversity in wild plant populations.
Microsatellite genotypesGenotype information from 11 microsatellite loci
(Hefo1, Hefo4, Hefo10, Hefo2, Hefo11, Hefo13, Hefo9, Hefo8, Hefo7, Hefo3,
Hefo5) for individual Helleborus foetidus plants
sampledMicrosatellite.genotypes.txtGeographical coordinates of individual
Helleborus foetidus plants sampledGeographical.coordinates.xls
Sierra de Cazorla
Jaén province
Spain