10.5061/DRYAD.GTHT76HGW
Torazo, Ikeda
0000-0002-2958-5327
Hokkaido Research Organization
Kentaro, Mishima
Forestry and Forest Products Research Institute
Katsuhiko, Takata
Akita Prefectural University
Nobuhiro, Tomaru
Nagoya University
The origin and genetic variability of vegetatively propagated clones
identified from old planted trees and plantations of Thujopsis dolabrata
var. hondae in Ishikawa Prefecture, Japan
Dryad
dataset
2019
2019-10-31T00:00:00Z
2019-10-31T00:00:00Z
en
88881 bytes
3
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Clonal plantations of Thujopsis dolabrata var. hondae have been
established in Ishikawa Prefecture, Japan, since at least the 1800s.
Historical planting of the species has led to the development of
vegetatively propagated local cultivars, which originated from ‘donor’
trees that have often been conserved in sacred groves or avenues at
shrines and temples. These donor trees must have been selected from
natural populations. In this study we estimated the origin and genetic
variability of clones identified among old planted trees and clonal
plantations of T. dolabrata var. hondae, using 19 microsatellite markers.
We discovered 12 clones among old planted trees, including five identical
to members of a set of 14 we previously identified in plantations (giving
21 clones in total). Based on analyses combining assignment and exclusion
tests, we inferred origins of eight of those 21 clones: six may have
originated from a natural population distributed in Ishikawa, one from
Hokkaido & Aomori, and the other from Iwate & Yamagata,
suggesting the clones constituting cultivars have multiple origins. The
clones identified in plantations have significantly lower genetic
variability, and higher relatedness, indicating that clones of cultivars
have a much narrower genetic base than those of natural populations. We
suggest new clones selected from natural populations elsewhere, as well as
Ishikawa, are needed for future breeding of T. dolabrata var. hondae to
develop clonal forestry for this species.
Genotypes of 410 individuals of Thujopsis dolabrata var. hondae at 19
microsatellite loci described in the cited study. Tissue was collected
from 17 natural populations in Japan.
ikeda_et_al_Thujopsis_dolabrata_var_hondae_17_nanural_populations_19_loci_SSRs_GENOTYPE_TABLES_Tree_Genetics_Genomes.xlsx