10.5061/DRYAD.GS835
Lehnert, Sarah J.
University of Windsor
Heath, Daniel D.
University of Windsor
Devlin, Robert H.
Fisheries and Oceans Canada
Pitcher, Trevor E.
University of Windsor
Data from: Post-spawning sexual selection in red and white Chinook salmon
(Oncorhynchus tshawytscha)
Dryad
dataset
2016
Oncorhynchus tshawytscha
competitive fertilization
color polymorphism
Carotenoids
2016-08-18T15:47:28Z
2016-08-18T15:47:28Z
en
https://doi.org/10.1093/beheco/arw142
133967 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Post-copulatory processes, including sperm competition and cryptic female
choice (CFC), can play important roles in the maintenance of
polymorphisms. In Chinook salmon (Oncorhynchus tshawytscha), color morphs
(red and white) exist due to genetic polymorphisms affecting carotenoid
deposition in flesh, skin, and gametes. We investigated the role of
post-spawning sexual selection in maintaining the polymorphism in a mixed
population. First, we compared sperm velocity differences in water between
morphs. Next, we measured color-based CFC via 2 methods: 1) sperm velocity
in ovarian fluid and 2) in vitro competitive fertilization using paired
red and white males. We found that red males had marginally faster sperm
relative to white males in water, suggesting that carotenoid storage may
affect sperm performance. However, ovarian fluid of red and white females
influenced sperm velocity of red and white males differently, indicative
of color-based CFC on sperm velocity. Furthermore, we found evidence of
color-based CFC on paternity success during in vitro competitive
fertilizations; however, sperm velocity in ovarian fluid did not predict
results found under in vitro fertilization. Instead, in our study, sperm
velocity in water was a significant predictor of fertilization success.
When we accounted for this difference in sperm velocity (in water) between
paired males, we partitioned the amount of variation in fertilization
success that was attributed to individual level CFC (male pair × female)
and male competitiveness (male pair) as 43% and 16%, respectively. In
conclusion, post-spawning sexual selection processes represent important
mechanisms contributing to the maintenance of the color polymorphism in
nature.
SpermVelocityWater_Lehnert-BESperm velocity (VAP, VCL, VSL) of red and
white Chinook salmon males at 5 seconds post-activation in water. Each
male is represented by replicate velocity measures (if
applicable).SpermVelocityOF_Lehnert-BESperm velocity (VAP, VSL, VCL) and
sperm path straightness (STR) of red and white Chinook salmon males when
sperm is activated in ovarian fluid of red and white females. Sperm
metrics were recorded at 5 seconds post-activation in ovarian
fluid.PaternityCompetitiveFertilizations_Lehnert-BEPaternity assignment
for competitive fertilizations in red and white Chinook salmon. For each
competitive fertilization, the number of red sired and white sired eggs
are provided Difference between paired males is sperm velocity (VAP) in
water and ovarian fluid at 5 seconds post-activation are provided, as well
as the sperm density ratio (red:white sperm cells) within each competitive
fertilization.
Canada
Quesnel River
British Columbia