10.5061/DRYAD.GS7FV
Chubanov, Vladimir
Ludwig Maximilian University of Munich
Gudermann, Thomas
German Center for Lung Research
German Centre for Cardiovascular Research
Ludwig Maximilian University of Munich
Jonas, Wenke
German Center for Diabetes Research
German Institute of Human Nutrition
Schürmann, Annette
German Center for Diabetes Research
German Institute of Human Nutrition
Yildirim, Önder A
German Center for Lung Research
Shymkiv, Yuriy
Princeton Medical Institute
Ryazanov, Alexey G
Princeton Medical Institute
Weber, Christian
German Centre for Cardiovascular Research
Ludwig Maximilian University of Munich
van der Vorst, Emiel PC
Ludwig Maximilian University of Munich
Bartsch, Harald
Ludwig Maximilian University of Munich
Sotlar, Karl
Ludwig Maximilian University of Munich
Braun, Attila
University of Würzburg
Simmons, David G
University of Queensland
Ferioli, Silvia
Ludwig Maximilian University of Munich
Wisnowsky, Annika
Ludwig Maximilian University of Munich
Akdogan, Banu
Ludwig Maximilian University of Munich
Mittermeier, Lorenz
Ludwig Maximilian University of Munich
Sytik, Ludmila
Ludwig Maximilian University of Munich
Zierler, Susanna
Ludwig Maximilian University of Munich
Jurinovic, Vindi
Ludwig Maximilian University of Munich
Data from: Epithelial magnesium transport by TRPM6 is essential for
prenatal development and adult survival
Dryad
dataset
2017
Liver
Mg2+
Serum
TRPM6
2017-12-15T00:00:00Z
2017-12-15T00:00:00Z
en
https://doi.org/10.7554/elife.20914
4567554 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Mg2+ regulates many physiological processes and signalling pathways.
However, little is known about the mechanisms underlying the organismal
balance of Mg2+. Capitalizing on a set of newly generated mouse models, we
provide an integrated mechanistic model of the regulation of organismal
Mg2+ balance during prenatal development and in adult mice by the ion
channel TRPM6. We show that TRPM6 activity in the placenta and yolk sac is
essential for embryonic development. In adult mice, TRPM6 is required in
the intestine to maintain organismal Mg2+ balance, but is dispensable in
the kidney. Trpm6 inactivation in adult mice leads to shortened lifespan,
growth deficit and metabolic alterations indicative of impaired energy
balance. Dietary Mg2+ supplementation not only rescues all phenotypes
displayed by Trpm6-deficient adult mice, but also may extend the lifespan
of wildtype mice. Hence, maintenance of organismal Mg2+ balance by TRPM6
is crucial for prenatal development and survival to adulthood.
Whole genome profiling of hepatic transcripts altered in Trpm6-deficient
miceExcel file contains two worksheets: (1) Genome-wide analysis of
hepatic transcriptome in control vs Trpm6-deficient mice. (2) Up- and
down-regulated transcripts in the liver of Trpm6-deficient mice with the
false discovery rate (FDR) P≤0.1.Table S2.xlsIngenuity Pathway Analysis
(IPA) analysis of hepatic transcripts altered in Trpm6-deficient miceExcel
file contains two worksheets: (1) IPA Canonical Pathways representing
differentially expressed genes in the liver of Trpm6-deficient mice. (2)
IPA Causal Networks for differentially expressed genes in the liver of
Trpm6-deficient mice.Table S3.xlsMetabolic profiling of the serum, liver
and gastrocnemius muscle of Trpm6-deficient miceExcel file contains seven
worksheets: (1) Statistical analysis of metabolite measurements in serum
samples. (2) Statistical analysis of metabolite measurements in
gastrocnemius muscle samples. (3) Statistical analysis of metabolite
measurements in liver samples. (4) Metabolites significantly changed in
serum samples of Trpm6-deficient mice (FDR P≤0.05). (5) Metabolites
significantly changed in gastrocnemius muscle samples of Trpm6-deficient
mice (FDR P≤0.05). (6) Metabolites significantly changed in liver samples
of Trpm6-deficient mice (FDR P≤0.05). (7) Abbreviations of
metabolites.Table S4.xlsxMATLAB codeMATLAB code used for statistical
analysis of survival distributions of control and dietary treated
miceMATLAB Code.docx