10.5061/DRYAD.GJ8JV50
Mutschler, Hannes
MRC Laboratory of Molecular Biology
Taylor, Alexander I.
MRC Laboratory of Molecular Biology
Porebski, Benjamin T.
MRC Laboratory of Molecular Biology
Lightowlers, Alice
MRC Laboratory of Molecular Biology
Houlihan, Gillian
MRC Laboratory of Molecular Biology
Abramov, Mikhail
KU Leuven
Herdewijn, Piet
KU Leuven
Holliger, Philipp
MRC Laboratory of Molecular Biology
Data from: Random-sequence genetic oligomer pools display an innate
potential for ligation and recombination
Dryad
dataset
2018
2018-11-27T14:52:08Z
2018-11-27T14:52:08Z
en
https://doi.org/10.7554/eLife.43022
262134355 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Recombination, the exchange of information between different genetic
polymer strands, is of fundamental importance in biology for genome
maintenance and genetic diversification mediated by dedicated recombinase
enzymes. Here, we describe a pervasive non-enzymatic capacity for
recombination (and ligation) in random-sequence genetic oligomer pools.
Specifically, we examine random and semi-random eicosamer (N20) pools of
RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-)
and AtNA (altritol-nucleic acids). While DNA, ANA and HNA pools proved
inert, RNA and AtNA pools displayed diverse modes of spontaneous
intermolecular recombination, connecting recombination mechanistically to
the vicinal ring cis-diol configuration shared by RNA and AtNA. Thus, the
chemical constitution that renders both susceptible to hydrolysis emerges
as the fundamental determinant of an innate capacity for recombination,
which is shown to enable a concomitant increase in compositional,
informational and structural pool complexity and hence evolutionary
potential.
Sequencing files for Figure 1Fig1.zipSequencing files for Figure
4Fig4.zipSequencing files for Figure 5Fig5.zipSequencing files for Figure
6Fig6.zipData-sets used to identify motifs J4 and
H4motif_search.zipSequencing data for starting pools used in RNA ligation
/ recombination experimentspre-ligation.zip