10.5061/DRYAD.GH317
Roy, Virginie
Paris 12 Val de Marne University
Constantino, Reginaldo
University of Brasília
Chassany, Vincent
Paris 12 Val de Marne University
Giusti-Miller, Stephanie
Paris 12 Val de Marne University
Diouf, Michel
Paris 12 Val de Marne University
Mora, Philippe
Paris 12 Val de Marne University
Harry, Myriam
French National Centre for Scientific Research
Data from: Species delimitation and phylogeny in the genus Nasutitermes
(Termitidae: Nasutitermitinae) in French Guiana
Dryad
dataset
2013
ABGD
GMYC
COII
16S rDNA
ITS2
Nasutitermes
2013-12-18T21:26:42Z
2013-12-18T21:26:42Z
en
https://doi.org/10.1111/mec.12641
245626 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Species delimitation and identification can be arduous for taxa whose
morphologic characters are easily confused, which can hamper global
biodiversity assessments and pest species management. Exploratory methods
of species delimitation that use DNA sequence as their primary information
source to establish group membership and estimate putative species
boundaries are useful approaches, complementary to traditional taxonomy.
Termites of the genus Nasutitermes make interesting models for the
application of such methods. They are dominant in Neotropical primary
forests but also represent major agricultural and structural pests.
Despite the prevalence, pivotal ecological role and economical impact of
this group, the taxonomy of Nasutitermes species mainly depends on
unreliable characters of soldier external morphology. Here, we generated
robust species hypotheses for 79 Nasutitermes colonies sampled throughout
French Guiana without any a priori knowledge of species affiliation.
Sequence analysis of the mitochondrial cytochrome oxidase II gene was
coupled with exploratory species-delimitation tools, using the automatic
barcode gap discovery method (ABGD) and a generalized mixed
Yule-coalescent model (GMYC) to propose primary species hypotheses (PSHs).
PSHs were revaluated using phylogenetic analyses of two more loci
(mitochondrial 16S rDNA and nuclear internal transcribed spacer 2) leading
to 16 retained secondary species hypotheses (RSSH). Seven RSSHs,
represented by 44/79 of the sampled colonies, were morphologically
affiliated to species recognized as pests in the Neotropics, where they
represent a real invasive pest potential in the context of growing
ecosystem anthropization. Multigenic phylogenies based on combined
alignments (1426–1784 bp) were also reconstructed to identify ancestral
ecological niches and major-pest lineages, revealing that Guyanese pest
species do not form monophyletic groups.
Nasutitermes and outgroup COII haplotypesCytochrome oxidase II (COII)
unique haplotypes for Nasutitermes samples and two outgroups. Genbank
Accession Numbers and putative species are
indicated.COIIhaplotypes.fasNasutitermes and outgroup 16S haplotypes16S
rDNA (16S) unique haplotypes for Nasutitermes samples and two outgroups.
Genbank Accession Numbers and putative species are
indicated.16Shaplotypes.FASNasutitermes and outgroup ITS2
sequencesInternal Transcribed Spacers 2 (ITS2) sequences for Nasutitermes
samples and two outgroups. Genbank Accession Numbers and putative species
are indicated.ITS2_uipacconsensus.FASTotal combined alignment
(COII/16S/ITS2)Total combined alignment (1784bp) for COII, 16rDNA and ITS2
sequences. Samples with at least one missing locus were discarded from the
analyses, resulting in datasets of 64 Nasutitermes and two outgroup
samples.CONCATALL.fasMorphological datasetTaxonomic notes on the
identification of the Nasutitermes speciesmorpho.docx
French Guiana