10.5061/DRYAD.G0N2D
Yu, Hao
University of Colorado Boulder
Siewny, Matthew G. W.
University of Colorado Boulder
Edwards, Devin T.
University of Colorado Boulder
Sanders, Aric W.
National Institute of Standards and Technology
Perkins, Thomas T.
University of Colorado Boulder
Data from: Hidden dynamics in the unfolding of individual
bacteriorhodopsin proteins
Dryad
dataset
2018
single-molecule force spectroscopy
Biophysics
bacteriorhodopsin
membrane protein folding
National Science Foundation
https://ror.org/021nxhr62
DBI-135398; Phys-1125844
2018-02-10T00:00:00Z
2018-02-10T00:00:00Z
en
https://doi.org/10.1126/science.aah7124
4171964533 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Protein folding occurs as a set of transitions between structural states
within an energy landscape. An oversimplified view of the folding process
emerges when transiently populated states are undetected because of
limited instrumental resolution. Using force spectroscopy optimized for
1-microsecond resolution, we reexamined the unfolding of individual
bacteriorhodopsin molecules in native lipid bilayers. The experimental
data reveal the unfolding pathway in unprecedented detail. Numerous newly
detected intermediates—many separated by as few as two or three amino
acids—exhibited complex dynamics, including frequent refolding and state
occupancies of <10 μs. Equilibrium measurements between such states
enabled the folding free-energy landscape to be deduced. These results
sharpen the picture of the mechanical unfolding of membrane proteins and,
more broadly, enable experimental access to previously obscured protein
dynamics.
Fig 1CFig1C_figS6A.csvFig 1DFig1D.csvFig 1EFig1E.csvFig 1FFig1F.csvFig
2Fig 3Figure 3 and Fig S8A.csvFig 4ABFig4AB.csvFig 4CFig4C.csvFig
S1AFigS1A.csvFig S1BFigS1B.csvFig S2AFig S2BFig S2CFig S2DFig S2EFig
S2FFig S2GFig S2HFig S2IFig S2JFig S2KFig S2LFig S2MFig S2NFig
S3AFigS3A.csvFig S3BFigS3B.csvFig S3CFigureS3C.csvFig S4AFig S4BFig
S5FigS5.csvFig S6AFig1C_figS6A.csvFig S6BFigS6B.csvFig S6CFigS6C.csvFig
S6DFigS6D.csvFig S6EFigS6E.csvFig S7Fig S8AFigure 3 and Fig S8A.csvFig
S8BCFig S7 and S8BC.xlsxFig S9AFigS9A.csvFig S9BFigS9B.csvFig
S9CFigS9C.csvFig S9DFigS9D.csvFig S10AFigS10A.csvFig S10BFigS10B.csvFig
S10CFigS10C.csvFig S11AFigS11A.csvFig S11BFigS11B.csvFig
S13AFigS13A.csvFig S13BFigS13B.csvFig S13CFigS13C.csv