10.5061/DRYAD.FQ134
Oliveira, Marisa M.
Instituto Gulbenkian de Ciência
Shingleton, Alexander W.
Michigan State University
Mirth, Christen K.
Instituto Gulbenkian de Ciência
Data from: Coordination of wing and whole body development at
developmental milestones ensures robustness against environmental and
physiological perturbations
Dryad
dataset
2015
larval development
progression of pattern
developmental coordination
staging scheme
developmental robustness
developmental milestones
wing imaginal discs
2015-04-24T00:00:00Z
2015-04-24T00:00:00Z
en
https://doi.org/10.1371/journal.pgen.1004408
46794 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Development produces correctly patterned tissues under a wide range of
conditions that alter the rate of development in the whole body. We
propose two hypotheses through which tissue patterning could be
coordinated with whole body development to generate this robustness. Our
first hypothesis states that tissue patterning is tightly coordinated with
whole body development over time. The second hypothesis is that tissue
patterning aligns at developmental milestones. To distinguish between our
two hypotheses, we developed a staging scheme for the wing imaginal discs
of Drosophila larvae using the expression of canonical patterning genes,
linking our scheme to three whole body developmental events, moulting,
larval wandering and pupariation. We used our scheme to explore how the
progression of pattern changes when developmental time is altered either
by changing temperature or by altering the timing of hormone synthesis
that drives developmental progression. We found the expression pattern in
the wing disc always aligned at moulting and pupariation, indicating that
these key developmental events represent milestones. Between these
milestones, the progression of pattern showed greater variability in
response to changes in temperature and alterations in physiology.
Furthermore, our data showed that discs from wandering larvae had greater
variability in their patterning stage. Thus, for wing disc patterning
wandering does not appear to be a developmental milestone. Our findings
reveal that tissue patterning remains robust against environmental and
physiological perturbations by aligning at developmental milestones.
Furthermore, our work provides an important glimpse into how the
development of individual tissues is coordinated with the body as a whole.
Oliveira et al DataData and R-scripts for analyzing data.