10.5061/DRYAD.F3KC0
Shiver, Anthony L.
University of California System
Osadnik, Hendrik
University of California System
Kritikos, George
European Molecular Biology Laboratory
Li, Bo
University of North Carolina
Krogan, Nevan
QB3
University of California System
Gladstone Institutes
Typas, Athanasios
European Molecular Biology Laboratory
Gross, Carol A.
University of California System
QB3
Data from: A chemical-genomic screen of neglected antibiotics reveals
illicit transport of kasugamycin and blasticidin S
Dryad
dataset
2017
Illicit Transport
Chemical genomics
2017-06-08T00:00:00Z
2017-06-08T00:00:00Z
en
https://doi.org/10.1371/journal.pgen.1006124
10525648594 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Fighting antibiotic resistance requires a deeper understanding of the
genetic factors that determine the antibiotic susceptibility of bacteria.
Here we describe a chemical-genomic screen in Escherichia coli K-12 that
was designed to discover new aspects of antibiotic resistance by focusing
on a set of 26 antibiotics and other stresses with poorly characterized
mode-of-action and determinants of resistance. We show that the screen
identifies new resistance determinants for these antibiotics including a
common signature from two antimicrobials, kasugamycin and blasticidin S,
used to treat crop diseases like rice blast and fire blight. Following
this signature, we further investigated the mechanistic basis for
susceptibility to kasugamycin and blasticidin S in E. coli using both
genetic and biochemical approaches. We provide evidence that these
compounds hijack an overlapping set of peptide ABC-importers to enter the
bacterial cell. Loss of uptake may be an underappreciated mechanism for
the development of kasugamycin resistance in bacterial plant pathogens.
Metadata for the chemical genomics plates.Metadata for the plates used in
the chemical genomics screen, including uid, image analysis filename,
condition and concentration of antibiotic, source plate, flags, and plate
opacity IQR.ImageKeyFinal.xlsIris Image Analysis FilesetTar ball of all
Iris files generated in the current screen, see the image key for
associated metadata.irisfile.tarimagefileTar ball of plate images, see
image key for associated metadataInput Metadata for Statistical
AnalysisThese files include the metadata for conditions and arrays used as
input for the statistical analysis and generation of the fitness
score.InputFiles.tar10C fitness-scoresGene deletions identified by ECK
number. Ranked list of fitness-scores at 10C.10C.rnkCOG assignmentsCOG
category assignments for KEIO genes, for use in GSEA analysiskeioCOG.gmx