10.5061/DRYAD.D7WM37PZ2
Apirajkamol, Nonthakorn (Beatrice)
Australian National University
James, Bill
Commonwealth Scientific and Industrial Research Organisation
Gordon, Karl
Commonwealth Scientific and Industrial Research Organisation
Walsh, Tom
Commonwealth Scientific and Industrial Research Organisation
McGaughran, Angela
Australian National University
Data from: Oxidative stress delays development and alters gene expression
in the agricultural pest moth, Helicoverpa armigera
Dryad
dataset
2020
Chronic
Hormesis
Invertebrate
RNAseq
Helicoverpa armigera conferta
Australian Research Council
https://ror.org/05mmh0f86
DE160100685
Centre for Biodiversity Analysis; Australian National University*
Ignition Grant
Commonwealth Scientific and Industrial Research Organisation
https://ror.org/03qn8fb07
n/a
Centre for Biodiversity Analysis; Australian National University
Ignition Grant
2021-04-19T00:00:00Z
2021-04-19T00:00:00Z
en
119969 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Stress is a widespread phenomenon that all organisms must endure. Common
in nature is oxidative stress, which can interrupt cell homeostasis to
cause cell damage and may be derived from respiration or from
environmental exposure through diet. As a result of the routine exposure
from respiration, many organisms can mitigate the effects of oxidative
stress, but less is known about responses to oxidative stress from other
sources. Helicoverpa armigera is a major agricultural pest moth that
causes significant damage to crops worldwide. Here, we examined the
effects of oxidative stress on H. armigera by chronically exposing
individuals to paraquat - a free radical producer - and measuring changes
in development (weight, developmental rate, lifespan), and gene
expression. We found that oxidative stress strongly affected development
in H. armigera, with stressed samples spending more time as caterpillars
than control samples (>24 vs. ~15 days, respectively) and living
longer overall. We found 1,618 up- and 761 down-regulated genes,
respectively, in stressed vs. control samples. In the up-regulated gene
set was an over-representation of biological processes related to cuticle
and chitin development, glycine metabolism, and oxidation-reduction.
Oxidative stress clearly impacts physiology and biochemistry in H.
armigera and the interesting finding of an extended lifespan in stressed
individuals could demonstrate hormesis, the process whereby toxic
compounds can actually be beneficial at low doses. Collectively, our
findings provide new insights into physiological and gene expression
responses to oxidative stress in invertebrates.
We examined the effects of oxidative stress on Helicoverpa armigera by
chronically exposing individuals to paraquat - a free radical producer -
and measuring changes in development (weight, developmental rate,
lifespan), and gene expression. Please see the manuscript for full
details.