10.5061/DRYAD.D7WM37PXC
Loureiro, Livia O.
0000-0003-0098-7901
Hospital for Sick Children
Engstrom, Mark D.
Royal Ontario Museum
Lim, Burton K.
0000-0002-0884-0421
Royal Ontario Museum
Data from: Comparative phylogeography of mainland and insular species of
Neotropical molossid bats (Molossus)
Dryad
dataset
2019
2019-11-27T00:00:00Z
2019-11-27T00:00:00Z
en
6609252 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Genetic structures, historical events, habitat preferences, and geographic
barriers might result in distinct patterns in insular versus mainland
populations. Comparison between these two biogeographic systems provides
an opportunity to investigate the relative role of isolation in
phylogeographic patterns and to elucidate the importance of evolution and
demographic history in population structure. Herein we use a genotype by
sequencing approach (GBS) to explore population structure within three
species of mastiff bats (Molossus molossus, M. coibensis, and M. milleri),
which represent different ecological histories and geographical
distributions in the genus to explore phylogeographic patterns and better
understand the role of geographic barriers in their dispersal and gene
flow. We tested the hypotheses that oceanic straits serve as barriers to
dispersal in Caribbean bats and that isolated island populations are more
likely to experience genetic drift and bottlenecks relative to highly
connected ones, which should have different phylogeographic patterns. We
show that population structures vary according to general habitat
preferences, to levels of population isolation, and to historical
fluctuations in climate. In our dataset, mainland geographic barriers
played only a small role in isolation of lineages. However, oceanic
straits posed a partial barrier to the dispersal for some populations
within some species (M. milleri), but do not seem to disrupt gene flow in
others (M. molossus). Lineages on distant islands undergo genetic
bottlenecks more frequently than island lineages closer to the mainland,
which have a greater exchange of haplotypes.
We obtained tissue samples from 54 M. molossus from South America, Middle
America and the Lesser Antilles, 20 M. coibensis from South and Middle
America, and 19 M. milleri from the Greater Antilles. Samples description
and localities are described in Loureiro et al. (in press). Tissues
samples included skeletal muscle, liver, heart, and kidney and were
preserved in 95% ethanol or were frozen in liquid nitrogen upon collection
of the specimen in the field. DNA extraction was conducted using Qiagen
DNeasy extraction kit (Qiagen, Inc. Valencia, CA, USA) following standard
protocols. Genomic DNA quality was checked by visual inspection on an
agarose gel and the DNA concentration was measured using a Nanodrop
spectrophotometer (Nanodrop Techinologies). We used 30 ul of DNA samples
with concentrations higher than 100 ng/ul for library preparation and for
the genotyping by sequencing approach following the protocol described by
Elshire et al. (2011). All libraries were sequenced on an Illumina HiSeq
2000 in the Cornell Institute of Genomic Diversity (IGD). De novo
genotyping was performed using the Universal Network-Enable Analysis Kit
(UNEAK) pipeline, available on TASSEL 3.0 software (Bradbury et al.,
2007). Quality control and filtering of the reference genotypes of each
species sample were also conducted on TASSEL 3.0 (Loureiro et al., in
press). References Bradbury, P.J., Zhang, Z., Kroon, D.E., Casstevens,
T.M., Ramdoss, Y., Buckler, E.S., 2007. TASSEL: Software for association
mapping of complex traits in diverse samples. Bioinformatics 23,
2633–2635. https://doi.org/10.1093/bioinformatics/btm308 Elshire, R.J.,
Glaubitz, J.C., Sun, Q., Poland, J.A., Kawamoto, K., Buckler, E.S.,
Mitchell, S.E., 2011. A robust, simple genotyping-by-sequencing (GBS)
approach for high diversity species. PLoS One 6, 1–10.
https://doi.org/10.1371/journal.pone.0019379 Loureiro, L. O., Engstrom, M.
D., Lim, B. K. (In press) Next Comparative phylogeography of mainland and
insular species of Neotropical molossid bats (Molossus). Ecology and
Evolution. Submitted on July 25, 2019. 43p. ECE-2019-06-00727.
Genotype by Sequencing data on three species of Molossus (Molossidae,
Chiroptera)