10.5061/DRYAD.D1ND3
Ghosh, Shampa M.
Michigan State University
Testa, Nicholas D.
Michigan State University
Shingleton, Alexander W.
Michigan State University
Data from: Temperature-size rule is mediated by thermal plasticity of
critical size in Drosophila melanogaster
Dryad
dataset
2013
critical size
holometabolous insects
temperature size rule
growth duration
growth rate
2013-05-22T18:50:04Z
2013-05-22T18:50:04Z
en
https://doi.org/10.1098/rspb.2013.0174
108400 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Most ectotherms show an inverse relationship between developmental
temperature and body size, a phenomenon known as the temperature size rule
(TSR). Several competing hypotheses have been proposed to explain its
occurrence. According to one set of views, the TSR results from inevitable
biophysical effects of temperature on the rates of growth and
differentiation, whereas other views suggest the TSR is an adaptation that
can be achieved by a diversity of mechanisms in different taxa. Our data
reveal that the fruit fly, Drosophila melanogaster, obeys the TSR using a
novel mechanism: reduction of critical size at higher temperatures. In
holometabolous insects, attainment of critical size initiates the hormonal
cascade that terminates growth, and hence, Drosophila larvae appear to
instigate the signal to stop growth at a smaller size at higher
temperatures. This is in contrast to findings from another holometabolous
insect, Manduca sexta, in which the TSR results from the effect of
temperature on the rate and duration of growth. This contrast suggests
that there is no single mechanism that accounts for the TSR. Instead, the
TSR appears to be an adaptation that is achieved at a proximate level
through different mechanisms in different taxa.
Growth curve dataMetadata used for Figure 1, Figure 2 and supplementary
Figure 1. # The variables in the file include temp = rearing temperature
of flies age = hours from hatching stage = developmental stage weight =
weight of individuals in mggrowth.curve.data.csvCritical size dataMetadata
used for to calculate the critical size (Figure 2, Figure 3 &
supplementary figure 1) Variables in the file include: temp = rearing
temperature for the flies. larval = larval weight at starvation. pupariate
= pupariation status. ttp = time to pupariation from starvation in hours.
pupal = pupal weight. eclose = eclosion statuscsdata.csvCritical size
breakpoint R scriptR script for calculating using the breakpoint method.
The file includes scripts for finding the breakpoint in the plot of ttp
vs. larval weight at starvation, bootstrapping data and permutation
test.CS_breakpoint_R_script.pdf