10.5061/DRYAD.CK1QS
Kueneman, Jordan G.
University of Colorado Boulder
Woodhams, Douglas C.
University of Massachusetts Boston
Harris, Reid
James Madison University
Archer, Holly M.
University of Colorado Boulder
Knight, Rob
University of California, San Diego
McKenzie, Valerie J.
University of Colorado Boulder
Data from: Probiotic treatment restores protection against lethal fungal
infection lost during amphibian captivity
Dryad
dataset
2017
Anaxyrus boreas
captivity
amphibian
Batrachochytrium dendrobatidis
Probiotics
National Science Foundation
https://ror.org/021nxhr62
DEB 1146284, DEB 1136602
2017-12-28T20:51:04Z
2017-12-28T20:51:04Z
en
https://doi.org/10.1098/rspb.2016.1553
6561558810 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Host-associated microbiomes perform many beneficial functions including
resisting pathogens and training the immune system. Here, we show that
amphibians developing in captivity lose substantial skin bacterial
diversity, primarily due to reduced ongoing input from environmental
sources. We combined studies of wild and captive amphibians with a
database of over 1 000 strains that allows us to examine antifungal
function of the skin microbiome. We tracked skin bacterial communities of
62 endangered boreal toads, Anaxyrus boreas, across 18 time points, four
probiotic treatments, and two exposures to the lethal fungal pathogen
Batrachochytrium dendrobatidis (Bd) in captivity, and compared these to 33
samples collected from wild populations at the same life stage. As the
amphibians in captivity lost the Bd-inhibitory bacteria through time, the
proportion of individuals exposed to Bd that became infected rose from 33%
to 100% in subsequent exposures. Inoculations of the Bd-inhibitory
probiotic Janthinobacterium lividum resulted in a 40% increase in survival
during the second Bd challenge, indicating that the effect of microbiome
depletion was reversible by restoring Bd-inhibitory bacteria. Taken
together, this study highlights the functional role of ongoing
environmental inputs of skin-associated bacteria in mitigating a
devastating amphibian pathogen, and that long-term captivity decreases
this defensive function.
Captive Wild Environment
ComparisonCaptive_wild_enviroment_comparison_Dryad.csvMapping
FileMapping_MCE_7_MCE_8_Time_Treat_exp.txtOTU
Tableotu_table_RSPB20161553_Dryad.biomPlate_1_6_Frog_Undetermined_S0_L001_I1_001Plate_1-6_Frog_Undetermined_S0_L001_R1_001Plate_1-6_Frog_Undetermined_S0_L001_R2_001Plate_1_6_Frog_Undetermined_S0_L001_R2_001.fastq.gzPlates_7_12_Frog_Undetermined_S0_L001_I1_001Plates_7_12_Undetermined_S0_L001_R1_001Plates_7_12_Undetermined_S0_L001_R2_001
Colorado