10.5061/DRYAD.C6G84
Kartzinel, Tyler R.
University of Georgia
Trapnell, Dorset W.
University of Georgia
Shefferson, Richard P.
University of Georgia
Data from: Highly diverse and spatially heterogeneous mycorrhizal
symbiosis in a rare epiphyte is unrelated to broad biogeographic or
environmental features
Dryad
dataset
2013
Epidendrum firumum
phylogenetic community dissimilarity
Mycorrhizal symbiosis
Orchidaceae
Epiphyte
Sebacinaceae
Tulasnellaceae
current
2013-09-20T17:48:21Z
2013-09-20T17:48:21Z
en
https://doi.org/10.1111/mec.12536
595490 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Symbiotic interactions are common in nature. In dynamic or degraded
environments, the ability to associate with multiple partners (i.e. broad
specificity) may enable species to persist through fluctuations in the
availability of any particular partner. Understanding how species
interactions vary across landscapes is necessary to anticipate direct and
indirect consequences of environmental degradation on species
conservation. We asked whether mycorrhizal symbiosis by populations of a
rare epiphytic orchid (Epidendrum firmum) is related to geographic or
environmental heterogeneity. The latter would suggest that interactions
are governed by environmental conditions rather than historic isolation of
populations and/or mycorrhizal fungi. We used DNA-based methods to
identify mycorrhizal fungi from eleven E. firmum populations in Costa
Rica. We used molecular and phylogenetic analyses to compare associations.
Epidendrum firmum exhibited broad specificity, associating with diverse
mycorrhizal fungi, including six Tulasnellaceae molecular operational
taxonomic units (MOTUs), five Sebacinales MOTUs and others. Notably,
diverse mycorrhizal symbioses formed in disturbed pasture and roadside
habitats. Mycorrhizal fungi exhibited significant similarity within
populations (spatial and phylogenetic autocorrelation) and significant
differences among populations (phylogenetic community dissimilarity).
However, mycorrhizal symbioses were not significantly associated with
biogeographic or environmental features. Such unexpected heterogeneity
among populations may result from complex combinations of fine-scale
environmental factors and macro-evolutionary patterns of change in
mycorrhizal specificity. Thus, E. firmum exhibits broad specificity and
the potential for opportunistic associations with diverse fungi. We
suggest that these characteristics could confer symbiotic assurance when
mycorrhizal fungi are stochastically available, which may be crucial in
dynamic or disturbed habitats such as tropical forest canopies.
ASCO_FASTAS_ALIGNED_58S_OPTSILAlignment of 5.8S sequences corresponding to
ascomycetes used in clustering analysis by
OPTSIL.BASID_FASTAS_ALIGNED_58S_OPTSILAlignment of 5.8S sequences
corresponding to basidiomycetes used in clustering analysis by OPTSIL.
File is in FASTA format.TUL_ML56_ALNAlignment of mitochondrial large
subunit ribosomal DNA used in both clustering analysis by OPTSIL and
phylogenetic analysis. The file is in FASTA
format.Sebacinales_58sAlignment of Sebacinales 5.8S used for inferring
phylogeny. The file is in phylip format.Sebacinales_ITSAlignment of
Sebacinales sequences spanning ITS1-5.8S-ITS2 used in analysis of
molecular diversity. The file is in FASTA format.Sebacinales_treeThe tree
file for the Sebacinales phylogeny in analysis of phylogenetic variation.
The file is in newick format.Tulasnellaceae_treeThe tree file for the
Tulasnellaceae phylogeny in analysis of phylogenetic variation. The file
is in newick format.Tulasnellaceae_58sAlignment of Tulasnellaceae 5.8S
used for inferring the phylogeny. The file is in PHYLIP
format.Tulasnellaceae_ITSAlignment of Tulasnellaceae sequences spanning
ITS1-5.8S-ITS2 used in analysis of molecular variation. The alignment is
in FASTA format.Tulasnellaceae_MLAlignment of Tulasnellaceae mitochondrial
large subunit (mtLSU) used for inferring the phylogeny. The data file is
in PHYLIP format.Tulasnellaceae_MLtreeThe tree file for the phylogeny of
the Tulasnellaceae mitochondrial large subunit of rDNA (mtLSU). The file
is in newick format.
Costa Rica