10.5061/DRYAD.C289V
Rodríguez-Quilón, Isabel
Forest Research
Santos-del-Blanco, Luis
University of Lausanne
University of Valladolid
Serra-Varela, María Jesús
University of Valladolid
Forest Research
Koskela, Jarkko
Department of Forest Ecology and Genetics; Forest Research Centre;
INIA-CIFOR; Carretera A Coruña km 7.5 Madrid 28040 Spain
González-Martínez, Santiago César
University of Bordeaux
University of Valladolid
Forest Research
Alía, Ricardo
University of Valladolid
Forest Research
Data from: Capturing neutral and adaptive genetic diversity for
conservation in a highly structured tree species
Dryad
dataset
2016
Cuantitative genetics
nuclear microsatellites
SNPs
Dynamic conservation
Pinus pinaster Ait.
2016-04-20T12:42:10Z
2016-04-20T12:42:10Z
en
https://doi.org/10.1002/eap.1361
1577225 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Preserving intraspecific genetic diversity is essential for long-term
forest sustainability in a climate change scenario. Despite that, genetic
information is largely neglected in conservation planning, and how
conservation units should be defined is still heatedly debated. Here, we
use maritime pine (Pinus pinaster Ait.), an outcrossing long lived tree
with a highly fragmented distribution in the Mediterranean biodiversity
hotspot, to prove the importance of accounting for genetic variation - at
both neutral molecular markers and quantitative traits - to define useful
conservation units. Six gene pools associated to distinct evolutionary
histories were identified within the species using 12 microsatellites and
266 Single Nucleotide Polymorphisms (SNPs). In addition, height and
survival standing variation, their genetic control and plasticity were
assessed in a multisite clonal common garden experiment (16,544 trees). We
found high levels of quantitative genetic differentiation within
previously defined neutral gene pools. Subsequent cluster analysis and
post-hoc trait distribution comparisons allowed us to define ten
genetically homogeneous population groups with high evolutionary
potential. They constitute the minimum number of units to be represented
in a maritime pine dynamic conservation program. Our results uphold that
the identification of conservation units below the species level should
account for key neutral and adaptive components of genetic diversity,
especially in species with strong population structure and complex
evolutionary histories. The environmental zonation approach currently used
by the pan-European genetic conservation strategy for forest trees would
be largely improved by gradually integrating molecular and quantitative
trait information, as data become available.
Molecular marker dataMolecular marker data (genotyping results) for 266
Single Nucleotide Polimorphisms and 12 nuclear microsatellites for 517
maritime pine trees from 35 populations sampled across the species natural
range.MolMarker_Data_SNPs_nuSSRs.xlsxPhenotypic DataSurvival and total
height (cm) field measurements in four maritime pine common garden trials.
The four trials (Asturias, Portugal, Madrid and Cáceres) have the same
number of clones, with eight replicates per genotype, in a randomized
complete block design. The four trial sites provided contrasting
environmental conditions.Phenotypic_Data.xlsxClimatic dataClimatic data
(temperatures and rainfall) for each of the 35 maritime pine provenances
included in the study. Populations were sorted by neutral gene
pool.Climatic_Data.xlsx
Europe