10.5061/DRYAD.BRV15DVC4
Zane, Lorenzo
0000-0002-6963-2132
University of Padua
Marino, Ilaria Anna Maria
University of Padua
Schiavina, Marcello
Polytechnic University of Milan
Aglieri, Giorgio
Stazione Zoologica Anton Dohrn
Bevilacqua, Stanislao
University of Trieste
Boscari, Elisa
University of Padua
Congiu, Leonardo
University of Padua
Faggion, Sara
University of Padua
Kruschel, Claudia
University of Zadar
Papetti, Chiara
University of Padua
Patarnello, Tomaso
University of Padua
Paterno, Marta
University of Padua
Voutsinas, Emanuela
Hellenic Centre for Marine Research
Melià, Paco
Polytechnic University of Milan
Assessment of connectivity patterns of the marbled crab Pachygrapsus
marmoratus in the Adriatic and Ionian seas through combination of genetic
data and Lagrangian simulations
Dryad
dataset
2022
Lagrangian simulations
marbled crab
Microsatellites
seascape genetics
individual-based simulations
Pachygrapsus marmoratus
FOS: Natural sciences
European Commission
https://ror.org/00k4n6c32
287844
Ministry of Education, Universities and Research
https://ror.org/0166hxq48
Prot. 2020J3W3WC
2022-05-26T00:00:00Z
2022-05-26T00:00:00Z
en
https://doi.org/10.3389/fmars.2022.944851
130284 bytes
5
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Seascape connectivity studies, informing the level of exchange of
individuals between populations, can provide extremely valuable data for
marine population biology and conservation strategy definition. Here we
used a multidisciplinary approach to investigate the connectivity of the
marbled crab (Pachygrapsus marmoratus), a high dispersal species, in the
Adriatic and Ionian basins. A combination of genetic analyses (based on 15
microsatellites screened in 314 specimens), Lagrangian simulations
(obtained with a biophysical model of larval dispersal) and
individual-based forward-time simulations (incorporating species-specific
fecundity and a wide range of population sizes) disclosed the realized and
potential connectivity among eight different locations, including existing
or planned Marine Protected Areas (MPAs). Overall, data indicated a
general genetic homogeneity, after removing a single outlier locus
potentially under directional selection. Lagrangian simulations showed
that direct connections potentially exist between several sites, but most
sites did not exchange larvae. Forward-time simulations indicated that a
few generations of drift would produce detectable genetic differentiation
in case of complete isolation as well as when considering the direct
connections predicted by Lagrangian simulations.Overall, our results
suggest that the observed genetic homogeneity reflects a high level of
realized connectivity among sites, which might result from a regional
metapopulation dynamics, rather than from direct exchange among
populations of the existing or planned MPAs. Thus, in the Adriatic and
Ionian basins, connectivity might be critically dependent on unsampled,
unprotected, populations, even in species with very high dispersal
potential like the marbled crab. Our study pointed out the pitfalls of
using wide-dispersing species with broad habitat availability when
assessing genetic connectivity among MPAs or areas deserving protection
and prompts for the careful consideration of appropriate dispersing
features, habitat suitability, reproductive timing and duration in the
selection of informative species.
314 samples of Pachygrapsus marmoratus were collected in the Adriatic and
Ionian seas. Genomic DNA was extracted using a standard salting out
protocol. 15 Fifteen polymorphic microsatellite loci specific for P.
marmoratus were used for genetic analysis. Refer to the full article for
details at https://doi.org/10.3389/fmars.2022.944851
The "Pachygrapsus marmoratus_rawdata.txt" and "Pachygrapsus
marmoratus_genotypes.txt" files contain information on 15
microsatellite loci. Eight population samples are reported for a total of
314 individuals. Please note that locus pm108 was identifed as an outlier
using LOSITAN (Amos et al. 2008) and DETSEL (Vitalis et al. 2012). The
file "Pachygrapsus marmoratus_rawdata.txt" is a tab separated
text file, organized with a first header row identifying columns for
population ID, individual ID and names of the loci (two alleles per
locus). The following 314 rows report for each individual population ID,
individual ID, followed by genotypes information for each locus. For each
locus, the two corresponding columns report raw size in base pairs of each
of the two alleles. The file "Pachygrapsus
marmoratus_genotypes.txt" is a tab separated text file, organized
with a first header row identifying columns for population ID, individual
ID and names of the loci. The following 314 rows report for each
individual population ID, individual ID, followed by genotypes information
for each locus. Alleles are given in repeat numbers, after that binning
was performed automatically with the software FlexiBin version 2 (Amos et
al. 2007). Missing data are represented by '000'.