10.5061/DRYAD.B8GTHT7BX
Binks, Rachel
0000-0003-1851-730X
Department of Biodiversity, Conservation and Attractions
Steane, Dorothy
University of Tasmania
Byrne, Margaret
Department of Biodiversity, Conservation and Attractions
Genomic divergence in sympatry indicates strong reproductive barriers and
cryptic species within Eucalyptus salubris
Dryad
dataset
2021
2022-11-05T00:00:00Z
2022-11-05T00:00:00Z
en
https://doi.org/10.1007/s11295-015-0864-z
2516521 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Genetic studies are increasingly detecting cryptic taxa that likely
represent a significant component of global biodiversity. However, cryptic
taxa are often criticized because they are typically detected
serendipitously and may not receive the follow-up study required to verify
their geographic or evolutionary limits. Here, we follow-up a study of
Eucalyptus salubris that unexpectedly detected two divergent lineages but
was not sampled sufficiently to make clear interpretations. We undertook
comprehensive sampling for an independent genomic analysis (3,605 SNPs) to
investigate whether the two purported lineages remain discrete genetic
entities or if they intergrade throughout the species’ range. We also
assessed morphological and ecological traits, and sequenced chloroplast
DNA. SNP results showed strong genome-wide divergence (FST=0.252) between
two discrete lineages: one dominated the north and one the southern
regions of the species’ range. Within lineages gene flow was high, with
low differentiation (mean FST=0.056) spanning hundreds of kilometres. In
the central region, the lineages were interspersed but maintained their
genomic distinctiveness: an indirect demonstration of reproductive
isolation. Populations of the southern lineage exhibited significantly
lower specific leaf area and occurred on soils with lower phosphorus
relative to the northern lineage. Finally, two major chloroplast
haplotypes were associated with each lineage but were shared between
lineages in the central distribution. Together, these results suggest that
these lineages have non-contemporary origins and that ecotypic adaptive
processes strengthened their divergence more recently. We conclude that
these lineages warrant taxonomic recognition as separate species and
provide fascinating insight to eucalypt speciation.
Here, we present three datasets regarding SNP genotypes, specific leaf
area measurements and soil phosphorus content for a study of cryptic
species in Eucalyptus salubris. There is additional chloroplast sequence
data for this study available on GenBank: MT104517-MT104558 . 1. SNP
genotypes: this is the filtered dataset of 3605 SNP loci used in all
population genomic analyses. The data is in the format of a
2-row STRUCTURE input file with missing data coded as -9. 2. Specific
leaf area: individual measurements of specific leaf area (ratio of leaf
area to dry mass). Ten leaves per tree were measured across the same trees
sampled for DNA. Lineage classification is also indicated. 3. Soil
phosphorus: soil phosphorus content (mg/kg) for each of the sites sampled.
Lineage classification is also indicated.