10.5061/DRYAD.B8649
Ravago-Gotanco, Rachel June
de la Cruz, Talna Lorena
University of the Philippines Diliman
Pante, Ma. Josefa
University of the Philippines Diliman
Borsa, Philippe
Udayana University
Ravago-Gotanco, Rachel
University of the Philippines Diliman
Data from: Cryptic genetic diversity in the mottled rabbitfish Siganus
fuscescens with mitochondrial introgression at a contact zone in the South
China Sea
Dryad
dataset
2019
Genetic loci
Siganus fuscescens
Cryptic speciation
2019-02-12T00:00:00Z
2019-02-12T00:00:00Z
en
https://doi.org/10.1371/journal.pone.0193220
38201 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The taxonomy of the mottled rabbitfish Siganus fuscescens species complex
has long been challenging. In this study, we analyzed microsatellite
genotypes, mitochondrial lineages, and morphometric data from 373 S.
fuscescens individuals sampled from the northern Philippines and Hong Kong
(South China Sea, Philippine Sea and Sulu Sea basins), to examine putative
species boundaries in samples comprising three co-occurring mitochondrial
lineages previously reported to characterize S. fuscescens (Clade A and
Clade B) or S. canaliculatus (Clade C). We report the existence of two
cryptic species within S. fuscescens in the northeast region of the South
China Sea and northern Philippine Sea, supported by genetic and
morphological differences. Individual-based assignment methods recovered
concordant groupings of individuals into two nuclear genotype clusters
(Cluster 1, Cluster 2) with (1) limited gene flow, if any, between them
(FST = 0.241; P < 0.001); (2) low frequency of later-generation
hybrids; (3) significant association with mitochondrial Clade A and Clade
B, respectively; and (4) subtle yet significant body shape differences as
inferred from geometric morphometric analysis. The divergence between
mitochondrial Clade C and the two other clades was not matched by genetic
differences at microsatellite marker loci. The occurrence of discordant
mitonuclear combinations (20.5% of the total number of individuals) is
thought to result from mitochondrial introgression, consistent with a
scenario of demographic, and presumably spatial, post-Pleistocene
expansion of populations from northern regions into a secondary contact
zone in the South China Sea. Mitonuclear discordance due to introgression
obscures phylogenetic relationships for recently-diverged lineages, and
cautions against the use of mitochondrial markers alone for species
identification within the mottled rabbitfish species complex in the South
China Sea region.
Siganus fuscescens_Microsat_Genotypes_RRGotanco et al 2017Microsatellite
genotype data in csv format. Data collected from 12 microsatellite loci
for 343 individuals. File description in the first row. Locus names are in
the second row. Individual names are in the first column, followed by
alleles.Alleles are coded as fragment sizes, in separate columns. Missing
data are indicated by ‘?.’
Philippines