10.5061/DRYAD.9QV2K
Facette, Michelle R.
University of California, San Diego
Shen, Zhouxin
University of California, San Diego
Bjornsdotter, Fjola R.
University of California, San Diego
Briggs, Steven P.
University of California, San Diego
Smith, Laurie G.
University of California, San Diego
Data from: Parallel proteomic and phosphoproteomic analyses of successive
stages of maize leaf development
Dryad
dataset
2013
leaf / vegetative development
cell differentiation / specialization
cell expansion
Zea mays
Proteomics
2013-08-12T14:56:53Z
2013-08-12T14:56:53Z
en
https://doi.org/10.1105/tpc.113.112227
35749295 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
We carried out large-scale, quantitative analyses of the maize (Zea mays)
leaf proteome and phosphoproteome at four developmental stages. Exploiting
the developmental gradient of maize leaves, we analyzed protein and
phosphoprotein abundance as maize leaves transition from proliferative
cell division to differentiation to cell expansion, and compared these
developing zones to one another and the mature leaf blade. Comparison of
the proteomes and phosphoproteomes suggests a key role for
post-translational regulation in developmental transitions. Analysis of
proteins with cell wall- and hormone-related functions illustrates the
utility of the dataset and provides further insight into maize leaf
development. We compare phosphorylation sites identified here to those
previously identified in Arabidopsis. We also discuss instances where
comparison of phosphorylated and unmodified peptides from a particular
protein indicates tissue-specific phosphorylation. For example, comparison
of unmodified and phosphorylated forms of PIN1 suggests a tissue-specific
difference in phosphorylation, which correlates with changes in PIN1
polarization in epidermal cells during development. Together, our data
provide insights into regulatory processes underlying maize leaf
development, and provide a community resource cataloging the abundance and
phosphorylation status of thousands of maize proteins at four leaf
developmental stages.
tpc112227_Supplemental_Dataset1Unfiltered, unnormalized data for protein
identifications.tpc112227_Supplemental_Dataset28005 proteins and
corresponding NSAF vaues.tpc112227_Supplemental_Dataset3Normalized
Phosphopeptide nSPCs. Tab 1: Unfiltered list of all phosphopeptides
identified and their respective normalized spectral counts. Tab 2:
Phosphopeptides matched to protein
NSAFs.tpc112227_Supplemental_Dataset4Phosphoprotein normalized spectral
counts.tpc112227_Supplemental_Dataset5Motifs identified via Motif-X
surrounding localized phospho -serine, -threonine and -tyrosine
residues.tpc112227_Supplemental_Dataset6Individual peptides and
phosphopeptides used in this
study.tpc112227_Supplemental_Figs_TblsSupplemental Figures 1-4 and
Supplemental Tables
1-7tpc_1122277_Supplemental_FigsTbls.pdftpc_1122277_Supplemental_Tbl8