10.5061/DRYAD.9KP7D
Ruelens, Philip
KU Leuven
Zhang, Zhicheng
KU Leuven
van Mourik, Hilda
Wageningen University & Research
Maere, Steven
Flanders Institute for Biotechnology
Kaufmann, Kerstin
Wageningen University & Research
Geuten, Koen
KU Leuven
Data from: The origin of floral organ identity quartets
Dryad
dataset
2017
Flower development
MADS-domain
Ancestral Sequence Reconstruction
2017-11-28T00:00:00Z
2017-11-28T00:00:00Z
en
https://doi.org/10.1105/tpc.16.00366
985314 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The origin of flowers has puzzled plant biologists ever since Darwin
referred to their sudden appearance in the fossil record as an abominable
mystery. Flowers are considered to be an assembly of protective,
attractive and reproductive male and female leaf-like organs. Their origin
cannot be understood by a morphological comparison to gymnosperms, their
closest relatives, which develop separate male or female cones. Despite
these morphological differences, gymnosperms and angiosperms possess a
similar genetic toolbox consisting of phylogenetically related MADS-domain
proteins. Using ancestral MADS-domain protein reconstruction, we trace the
evolution of organ identity quartets along the stem lineage of crown
angiosperms. We provide evidence that current floral quartets specifying
male organ identity, which consist of four types of subunits, evolved from
ancestral complexes of two types of subunits through gene duplication and
integration of SEPALLATA proteins just before the origin of flowering
plants. Our results suggest that protein interaction changes underlying
this compositional shift were the result of a gradual and reversible
evolutionary trajectory. Modelling shows that such compositional changes
may have facilitated the evolution of the perfect, bisexual flower.
Supplemental Data Set 1List of accessions of SEPALLATA-like, APETALA3- and
PISTILLATA-like, AGAMOUS- and SEEDSTICK-like genes used for dating the
E-class duplication.SupplementalDataSet1.xlsxSupplemental Data Set
2Multiple alignment of AP3/PI used for estimating time of
diversification.SupplementalDataSet2Supplemental Data Set 3Multiple
alignment of AG/STK used for estimating time of
diversification.SupplementalDataSet3Supplemental Data Set 4Multiple
alignment of SEP3/SEP1 used for estimating time of
diversification.SupplementalDataSet4Supplemental Data Set 5List of
accession numbers of APETALA3- and PISTILLATA-like proteins, AG- and
STK-like proteins, and SEPALLATA-like proteins used for ancestral sequence
reconstruction.SupplementalDataSet5.xlsxSupplemental Data Set 6Multiple
alignment of AP3/PI used for ancestral protein
reconstruction.SupplementalDataSet6Supplemental Data Set 7Multiple
alignment of AG/STK used for ancestral protein
reconstruction.SupplementalDataSet7Supplemental Data Set 8Multiple
alignment of SEP3/SEP1 used for ancestral protein
reconstruction.SupplementalDataSet8