10.5061/DRYAD.9GHX3FFKT
Khurshid, Rubab
0000-0001-6542-817X
University of the Punjab
Naseer, Arooj
0000-0002-4458-9043
University of the Punjab
Ahmad, Israr
Women University of Azad Jammu and Kashmir, Bagh
Khalid, Abdual Nasir
University of the Punjab
ITS and LSU sequences, phylogenetic trees
Dryad
dataset
2022
FOS: Biological sciences
AJ&K
LSU
phylogeny
Russulaceae
2022-07-04T00:00:00Z
2022-07-04T00:00:00Z
en
https://doi.org/10.5281/zenodo.6675935
19675 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Russula kashmiriana sp. nov (subg. Tenellula, sect. Laricinae) has been
collected and described from Himalayan coniferous forest of Azad Jammu and
Kashmir (AJ&K), Pakistan. The taxon is characterized by bright
reddish orange pileus with cracked margins and obvious reddish orange
circle on the pileus, yellow context and light olive to yellowish amyloid
basidiospores. Micro and macroscopic features along with molecular
phylogenetic analysis based on large subunit (LSU) and internal
transcribed spacer (ITS) sequences data confirmed the status of Russula
kashmiriana as a distinct species. AJ&K, LSU, Phylogeny,
Russulaceae.
DNA was extracted from gills of dried fruiting bodies by modified CTAB
method and the rDNA ITS region was amplified using universal primer pair
ITS1F and ITS 4 (Gardes and Bruns, 1993; Bruns, 1995). The LSU regions
were amplified by using LR0R and LR5 primers (Ge et al., 2014). The PCR
products was sequenced using the same primers (Macrogen, Korea). To
generate consensus sequences of both LSU and ITS regions BioEdit version
7.2.5 (Hall, 1999) was used. Nucleotide sequence comparisons were
performed with Basic Local Alignment Search Tool (BLAST) network services
using National Center for Biotechnology Information (NCBI), USA database
(http://www.ncbi.nlm.nih.gov/). For phylogenetic tree construction and
alignment of sequences, closely related sequences were retrieved from
GenBank. Published sequences and data from the literature were also added
in final data set. An online software MUSCLE was used for alignment
purpose (http://www.ebi.ac.uk/Tools/msa/muscle/). Phylogenetic analyses
were performed in MEGA6.
BioEdit, MUSCLE and MEGA6.