10.5061/DRYAD.98S363K3
Spice, Erin K.
University of Manitoba
Goodman, Damon H.
United States Fish and Wildlife Service
Reid, Stewart B.
Western Fishes, 2045 East Main Street, Ashland, OR 97520, USA
Docker, Margaret F.
University of Manitoba
Data from: Neither philopatric nor panmictic: microsatellite and mtDNA
evidence suggests lack of natal homing but limits to dispersal in Pacific
lamprey
Dryad
dataset
2012
Entosphenus tridentatus
2012-02-27T21:57:55Z
2012-02-27T21:57:55Z
en
https://doi.org/10.1111/j.1365-294X.2012.05585.x
82285 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Most species with lengthy migrations display some degree of natal homing;
some (e.g., migratory birds and anadromous salmonids) show spectacular
feats of homing. However, studies of the sea lamprey (Petromyzon marinus)
indicate that this anadromous species locates spawning habitat based on
pheromonal cues from larvae rather than through philopatry. Previous
genetic studies in the anadromous Pacific lamprey (Entosphenus
tridentatus) have both supported and rejected the hypothesis of natal
homing. To resolve this, we used nine microsatellite loci to examine
population structure in 965 Pacific lamprey from 20 locations from central
British Columbia to southern California, and supplemented this analysis
with mitochondrial DNA restriction fragment length polymorphism analysis
on a subset of 530 lamprey. Microsatellite analysis revealed: 1)
relatively low but often statistically significant genetic differentiation
among locations (97% pairwise FST values were less than 0.04 but 73.7%
were significant); and 2) weak but significant isolation-by-distance (r2 =
0.0565, P = 0.0450) but no geographic clustering of samples. The few
moderate FST values involved comparisons with sites that were
geographically distant or far upstream. The mtDNA analysis—although
providing less resolution among sites (only 4.7% FST values were
significant)—was broadly consistent with the microsatellite results: 1)
the southernmost site and some sites tributary to the Salish Sea were
genetically distinct; and 2) southern sites showed higher haplotype and
private haplotype richness. These results are inconsistent with
philopatry, suggesting that anadromous lampreys are unusual among species
with long migrations, but suggest that limited dispersal at sea precludes
panmixia in this species.
Microsatellite allele sizes of Pacific lamprey from 20 locations along the
west coast of North AmericaMicrosatellite allele sizes determined using
Genemapper, formatted for Genepop.Genepop.txtList of haplotypes and
composite haplotypes for Pacific lamprey collected from 20 locations along
the west coast of North AmericaRFLP haplotypes determined by gel
electrophoresisRFLP results.txt