10.5061/DRYAD.975KK
Vereczkei, Andrea
Semmelweis University
Demetrovics, Zsolt
Eötvös Loránd University
Szekely, Anna
Eötvös Loránd University
Sarkozy, Peter
Antal, Peter
Szilagyi, Agnes
Semmelweis University
Sasvari-Szekely, Maria
Semmelweis University
Barta, Csaba
Semmelweis University
Data from: Multivariate analysis of dopaminergic gene variants as risk
factors of heroin dependence
Dryad
dataset
2013
candidate gene
Heroin
association study
DRD2
Dopamine
TaqIA SNP
DRD4
substance dependence
2013-07-01T17:07:58Z
2013-07-01T17:07:58Z
en
https://doi.org/10.1371/journal.pone.0066592
418292 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
BACKGROUND: Heroin dependence is a debilitating psychiatric disorder with
complex inheritance. Since the dopaminergic system has a key role in
rewarding mechanism of the brain, which is directly or indirectly targeted
by most drugs of abuse, we focus on the effects and interactions among
dopaminergic gene variants. OBJECTIVE: To study the potential association
between allelic variants of dopamine D2 receptor (DRD2), ANKK1 (ankyrin
repeat and kinase domain containing 1), dopamine D4 receptor (DRD4),
Catechol-O-methyl transferase (COMT) and dopamine transporter (SLC6A3)
genes and heroin dependence in Hungarian patients. METHODS: 303 heroin
dependent subjects and 555 healthy controls were genotyped for 7 single
nucleotide polymorphisms (SNPs): rs4680 of the COMT gene; rs1079597 and
rs1800498 of the DRD2 gene; rs1800497 of the ANKK1 gene; rs1800955,
rs936462 and rs747302 of the DRD4 gene. Four variable number of tandem
repeats (VNTRs) were also genotyped: 120 bp duplication and 48 bp VNTR in
exon 3 of DRD4 and 40 bp VNTR and intron 8 VNTR of SLC6A3. We also provide
a multivariate model for the associations among them implying Bayesian
networks in Bayesian multilevel analysis. FINDINGS AND CONCLUSIONS: In
single marker analysis the TaqIA (rs1800497) and TaqIB (rs1079597)
variants were associated with heroin dependence. Moreover, -521 C/T SNP
(rs1800955) of the DRD4 gene showed nominal association with a possible
protective effect of the C allele. After applying the Bonferroni
correction TaqIB was still significant suggesting that the minor (A)
allele of the TaqIB SNP is a risk component in the genetic background of
heroin dependence. The findings of the additional multiple marker analysis
are consistent with the results of the single marker analysis, but this
method was able to reveal an indirect effect of a promoter polymorphism
(rs936462) of the DRD4 gene and this effect is mediated through the -521
C/T (rs1800955) polymorphism in the promoter.
Vereczkei et al - Hungarian heroin303_control555Spreadsheet containing all
genotype-phenotype data on 303 Hungarian heroin dependent patients and 555
healthy controls including DRD2, DRD4, SLC6A3 (DAT1) and COMT gene
variants.
Hungary
Central-Europe