10.5061/DRYAD.935HP
Curry, Meghan
Paliulis, Leocadia
Welch, Kelton
Harwood, James
University of Kentucky
White, Jennifer
Data from: Multiple endosymbiont infections and reproductive manipulations
in a linyphiid spider population
Dryad
dataset
2014
Feminization
Wolbachia
reproductive manipulation
Mermessus fradeorum
multiple infection
endosymbiont
Rickettsia
Araneae
cytoplasmic incompatibility
Cardinium
2014-12-23T16:40:14Z
2014-12-23T16:40:14Z
en
https://doi.org/10.1038/hdy.2015.2
15301 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
In many arthropods, maternally inherited endosymbiotic bacteria can
increase infection frequency by manipulating host reproduction. Multiple
infections of different bacteria in a single host population are common,
yet few studies have documented concurrent endosymbiont phenotypes or
explored their potential interactions. We hypothesized that spiders might
be a particularly useful taxon for investigating endosymbiont
interactions, because they are host to a plethora of endosymbiotic
bacteria and frequently exhibit multiple infections. We established two
matrilines from the same population of the linyphiid spider Mermessus
fradeorum and then used antibiotic curing and controlled mating assays to
demonstrate that each matriline was subject to a distinct endosymbiotic
reproductive manipulation. One matriline was co-infected with Rickettsia
and Wolbachia and produced offspring with a radical female bias.
Antibiotic treatment eliminated both endosymbionts and restored an even
sex ratio to subsequent generations. Chromosomal and fecundity
observations suggest a feminization mechanism. In the other matriline, a
separate factorial mating assay of cured and infected spiders demonstrated
strong cytoplasmic incompatibility (CI) induced by a different strain of
Wolbachia. However, males with this Wolbachia induced only mild CI when
mated with the Rickettsia–Wolbachia females. In a subsequent survey of a
field population of M. fradeorum, we detected these same three
endosymbionts infecting 55% of the spiders in almost all possible
combinations, with nearly half of the infected spiders exhibiting multiple
infection. Our results suggest that a dynamic network of endosymbionts may
interact both within multiply infected hosts and within a population
subject to multiple strong reproductive manipulations.
Dryad Curry et al 2014 R1W1xW2 CI cross experimentRaw offspring hatching
data from experiment used to determine W2 males induce CI when mated with
R1W1 females in comparison to cured W2-males.Dryad Curry et al 2014
maternal symbiont effects on fitness parameters experiment.csvRaw data
from experiment used to identify effects of maternal endosymbionts on
basic life history parameters like fecundity and sex ratio.Dryad Curry et
al 2014 maternal symbiont effects on fitness parmaeters experiment.csv
.csvDryad Curry et al 2014 W2 Wolbachia CI experiment.csvRaw data from
factorial CI cross used to determine if W2 Wolbachia induces CI when mated
with uninfected W2- females.