10.5061/DRYAD.92C60
Harkess, Alex
University of Georgia
Mercati, Francesco
Institute of Biosciences and Bioresources
Shan, Hong-Yan
Chinese Academy of Sciences
Sunseri, Francesco
University of Reggio Calabria
Falavigna, Agostino
University of Georgia
Leebens-Mack, Jim
University of Georgia
Data from: Sex-biased gene expression in dioecious garden asparagus
(Asparagus officinalis)
Dryad
dataset
2015
garden asparagus
garden asparagus
sex chromosome
Asparagus officinalis
2015-06-23T16:41:57Z
2015-06-23T16:41:57Z
en
https://doi.org/10.1111/nph.13389
62057387 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Sex chromosomes have evolved independently in phylogenetically diverse
flowering plant lineages. The genes governing sex determination in
dioecious species remain unknown, but theory predicts that the linkage of
genes influencing male and female function will spur the origin and early
evolution of sex chromosomes. For example, in an XY system, the origin of
an active Y may be spurred by the linkage of female suppressing and male
promoting genes. Garden asparagus (Asparagus officinalis) serves as a
model for plant sex chromosome evolution, given that it has recently
evolved an XX/XY sex chromosome system. In order to elucidate the
molecular basis of gender differences and sex determination, we used
RNA-sequencing (RNA-Seq) to identify differentially expressed genes
between female (XX), male (XY) and supermale (YY) individuals. We
identified 570 differentially expressed genes, and showed that
significantly more genes exhibited male-biased than female-biased
expression in garden asparagus. In the context of anther development, we
identified genes involved in pollen microspore and tapetum development
that were specifically expressed in males and supermales. Comparative
analysis of genes in the Arabidopsis thaliana, Zea mays and Oryza sativa
anther development pathways shows that anther sterility in females
probably occurs through interruption of tapetum development before
microspore meiosis.
Asparagus officinalis transcriptome assemblyRaw garden asparagus
transcriptome assembly for Harkess et al. (2015). Transcripts were
digitally normalized to 30X and assembled in Trinity (r2012-10-05). All
raw RNA-seq reads can be found in NCBI BioProject
259909.Asparagus_officinalis.trinity.fa.bz2