10.5061/DRYAD.900
Gibson, Greg
North Carolina State University
Riley-Berger, Rebecca
North Carolina State University
Harshman, Larry
University of Nebraska - Lincoln
Kopp, Artyom
University of California, Davis
Vacha, Scott
Nuzhdin, Sergey
University of California, Davis
Wayne, Marta
University of Florida
Data from: Extensive sex-specific nonadditivity of gene expression in
Drosophila melanogaster
Dryad
dataset
2009
2009-10-03T04:48:02Z
2009-10-03T04:48:02Z
en
https://doi.org/10.1534/genetics.104.026583
34061508 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Assessment of the degree to which gene expression is additive and
heritable has important implications for understanding the maintenance of
variation, adaptation, phenotypic divergence, and the mapping of genotype
onto phenotype. We used whole-genome transcript profiling using Agilent
long-oligonucleotide microarrays representing 12,017 genes to demonstrate
that gene transcription is pervasively nonadditive in Drosophila
melanogaster. Comparison of adults of two isogenic lines and their
reciprocal F1 hybrids revealed 5820 genes as significantly different
between at least two of the four genotypes in either males or females or
across both sexes. Strikingly, while 25% of all genes differ between the
two parents, 33% differ between both F1's and the parents, averaged
across sexes. However, only 5% of genes show overdominance, suggesting
that heterosis for expression is rare.
SAS codeA Word file showing SAS-MAS code for the basic model and two
different sets of Difference estimatesAgilentCode.docMiame
ComplianceMIAMEglue.docSupplementary Table 1Expression and Classification
ResultsNG03_STable.zipRaw DataA .txt file containing all of the gene
sequences used in probe design by Agilent, extracted from the Drosophila
genome public release Version 3.0 (see
http://www.fruitfly.org/sequence/release3genomic.shtml for latest
version).DROSGENOME1_Agilent.zipGene SequencesAgilentNormalized.zip