10.5061/DRYAD.8S21M
Knebelsberger, Thomas
Senckenberg am Meer
Dunz, Andreas R.
Bavarian State Collection of Zoology
Neumann, Dirk
Bavarian State Collection of Zoology
Geiger, Matthias F.
Zoological Research Museum Alexander Koenig
Data from: Molecular diversity of Germany’s freshwater fishes and lampreys
assessed by DNA barcoding
Dryad
dataset
2014
BIN
2014-08-28T14:55:05Z
2014-08-28T14:55:05Z
en
https://doi.org/10.1111/1755-0998.12322
763954 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
This study represents the first comprehensive molecular assessment of
freshwater fishes and lampreys from Germany. We analysed COI sequences for
almost 80% of the species mentioned in the current German Red List. In
total, 1056 DNA barcodes belonging to 92 species from all major drainages
were used to (i) build a reliable DNA barcode reference library, (ii) test
for phylogeographic patterns, (iii) check for the presence of barcode gaps
between species and (iv) evaluate the performance of the barcode index
number (BIN) system, available on the Barcode of Life Data Systems. For
over 78% of all analysed species, DNA barcodes are a reliable means for
identification, indicated by the presence of barcode gaps. An overlap
between intra- and interspecific genetic distances was present in 19
species, six of which belong to the genus Coregonus. The Neighbour-Joining
phenogram showed 60 nonoverlapping species clusters and three singleton
species, which were related to 63 separate BIN numbers. Furthermore,
Barbatula barbatula, Leucaspius delineatus, Phoxinus phoxinus and Squalius
cephalus exhibited remarkable levels of cryptic diversity. In contrast, 11
clusters showed haplotype sharing, or low levels of divergence between
species, hindering reliable identification. The analysis of our barcode
library together with public data resulted in 89 BINs, of which 56% showed
taxonomic conflicts. Most of these conflicts were caused by the use of
synonymies, inadequate taxonomy or misidentifications. Moreover, our study
increased the number of potential alien species in Germany from 14 to 21
and is therefore a valuable groundwork for further faunistic
investigations.
GERFWF_Alignment_1056This alignment comprises 1056 DNA barcodes belonging
to 93 species of freshwater fishes and lampreys from Germany.