10.5061/DRYAD.8QV00
Readman, Gareth D.
University of Bristol
Owen, Stewart F.
Murrell, Joanna C.
University of Bristol
Knowles, Toby G.
University of Bristol
Data from: Do fish perceive anaesthetics as aversive?
Dryad
dataset
2013
Humane euthanasia
fish welfare
fish welfare
Danio rerio
Etomidate
anaesthesia
MS222
2013-09-27T14:27:58Z
2013-09-27T14:27:58Z
en
https://doi.org/10.1371/journal.pone.0073773
5680552 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
This study addresses a fundamental question in fish welfare: are the
anaesthetics used for fish aversive? Despite years of routine general use
of many agents, within both scientific research and aquaculture, there is
a paucity of information regarding their tolerance and associated
behavioural responses by fish. This study examined nine of the most
commonly used fish anaesthetic agents, and performed preference tests
using adult mixed sex zebrafish (Danio rerio), the most commonly held
laboratory fish. Video tracking software quantified swimming behaviour
related to aversion for each anaesthetic at 50% of its standard
recommended dose compared with clean water in a flow-through chemotaxic
choice chamber. Results suggest that several commonly used anaesthetics
were aversive, including two of the most commonly recommended and used:
MS222 (ethyl 3-aminobenzoate methanesulphate) and benzocaine. For ethical
best practice, it is recommended that compounds that are aversive, even at
low concentration, should no longer be used routinely for anaesthesia or
indeed the first step of humane euthanasia of adult zebrafish. Two agents
were found not to induce aversive behavioural responses; etomidate and
2,2,2 tribromoethanol. For the millions of adult zebrafish used in
laboratories and breeding worldwide, etomidate appears best suited for
future routine humane use.
222Offline analysis of the video recordings was carried out using
VideoTrack analysis software (Version 2.5.0.25, ViewPoint, Lyon, France).
The data output from VideoTrack was subsequently formatted in Excel
(Microsoft office, 2007) and covers the distance moved in exposed/un
exposed lanes, movements and time spent.2PE sinOffline analysis of the
video recordings was carried out using VideoTrack analysis software
(Version 2.5.0.25, ViewPoint, Lyon, France). The data output from
VideoTrack was subsequently formatted in Excel (Microsoft office, 2007)
and covers the distance moved in exposed/un exposed lanes, movements and
time spent.Benz sinOffline analysis of the video recordings was carried
out using VideoTrack analysis software (Version 2.5.0.25, ViewPoint, Lyon,
France). The data output from VideoTrack was subsequently formatted in
Excel (Microsoft office, 2007) and covers the distance moved in exposed/un
exposed lanes, movements and time spent.CleanOffline analysis of the video
recordings was carried out using VideoTrack analysis software (Version
2.5.0.25, ViewPoint, Lyon, France). The data output from VideoTrack was
subsequently formatted in Excel (Microsoft office, 2007) and covers the
distance moved in exposed/un exposed lanes, movements and time
spent.EthanolOffline analysis of the video recordings was carried out
using VideoTrack analysis software (Version 2.5.0.25, ViewPoint, Lyon,
France). The data output from VideoTrack was subsequently formatted in
Excel (Microsoft office, 2007) and covers the distance moved in exposed/un
exposed lanes, movements and time spent.Etom sinOffline analysis of the
video recordings was carried out using VideoTrack analysis software
(Version 2.5.0.25, ViewPoint, Lyon, France). The data output from
VideoTrack was subsequently formatted in Excel (Microsoft office, 2007)
and covers the distance moved in exposed/un exposed lanes, movements and
time spent.HCLOffline analysis of the video recordings was carried out
using VideoTrack analysis software (Version 2.5.0.25, ViewPoint, Lyon,
France). The data output from VideoTrack was subsequently formatted in
Excel (Microsoft office, 2007) and covers the distance moved in exposed/un
exposed lanes, movements and time spent.Iso sinOffline analysis of the
video recordings was carried out using VideoTrack analysis software
(Version 2.5.0.25, ViewPoint, Lyon, France). The data output from
VideoTrack was subsequently formatted in Excel (Microsoft office, 2007)
and covers the distance moved in exposed/un exposed lanes, movements and
time spent.LidocaineOffline analysis of the video recordings was carried
out using VideoTrack analysis software (Version 2.5.0.25, ViewPoint, Lyon,
France). The data output from VideoTrack was subsequently formatted in
Excel (Microsoft office, 2007) and covers the distance moved in exposed/un
exposed lanes, movements and time spent.MS222Pro sinOffline analysis of
the video recordings was carried out using VideoTrack analysis software
(Version 2.5.0.25, ViewPoint, Lyon, France). The data output from
VideoTrack was subsequently formatted in Excel (Microsoft office, 2007)
and covers the distance moved in exposed/un exposed lanes, movements and
time spent.Q S BufOffline analysis of the video recordings was carried out
using VideoTrack analysis software (Version 2.5.0.25, ViewPoint, Lyon,
France). The data output from VideoTrack was subsequently formatted in
Excel (Microsoft office, 2007) and covers the distance moved in exposed/un
exposed lanes, movements and time spent.