10.5061/DRYAD.8M201
Yano, C.F.
Federal University of São Carlos
Bertollo, L.A.C.
Federal University of São Carlos
Ezaz, T.
University of Canberra
Trifonov, V.
Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia
Sember, A.
Institute of Animal Physiology and Genetics
Liehr, T.
Jena University Hospital
Cioffi, M.B.
Federal University of São Carlos
Data from: Highly conserved Z and molecularly diverged W chromosomes in
the fish genus Triportheus (Characiformes, Triportheidae)
Dryad
dataset
2016
2016-08-09T15:36:12Z
2016-08-09T15:36:12Z
en
https://doi.org/10.1038/hdy.2016.83
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The main objectives of this study were to test: (1) whether the
W-chromosome differentiation matches to species’ evolutionary divergence
(phylogenetic concordance) and (2) whether sex chromosomes share a common
ancestor within a congeneric group. The monophyletic genus Triportheus
(Characiformes, Triportheidae) was the model group for this study. All
species in this genus so far analyzed have ZW sex chromosome system, where
the Z is always the largest chromosome of the karyotype, whereas the W
chromosome is highly variable ranging from almost homomorphic to highly
heteromorphic. We applied conventional and molecular cytogenetic
approaches including C-banding, ribosomal DNA mapping, comparative genomic
hybridization (CGH) and cross-species whole chromosome painting (WCP) to
test our questions. We developed Z- and W-chromosome paints from T.
auritus for cross-species WCP and performed CGH in a representative
species (T. signatus) to decipher level of homologies and rates of
differentiation of W chromosomes. Our study revealed that the ZW sex
chromosome system had a common origin, showing highly conserved Z
chromosomes and remarkably divergent W chromosomes. Notably, the W
chromosomes have evolved to different shapes and sequence contents within
~15–25 Myr of divergence time. Such differentiation highlights a dynamic
process of W-chromosome evolution within congeneric species of
Triportheus.
Sampling sites with coordinatesDescription of the sampling sites and their
correct GPS coordinatesSamplis sites with coordinates.doc