10.5061/DRYAD.8G5D2
Emerson, Brent C.
University of East Anglia
Casquet, Juliane
French National Centre for Scientific Research
López, Heriberto
Laboratory Evolution and Biological Diversity
Cardoso, Pedro
University of Helsinki
Borges, Paulo A. V.
University of La Laguna
Mollaret, Noémy
National Museum of Natural History
Oromí, Pedro
University of La Laguna
Strasberg, Dominique
University of La Réunion
Thébaud, Christophe
French National Centre for Scientific Research
Data from: A combined field survey and molecular identification protocol
for comparing forest arthropod biodiversity across spatial scales
Dryad
dataset
2016
biological species
Araneae
parataxonomy
barcode
2016-10-20T13:32:12Z
2016-10-20T13:32:12Z
en
https://doi.org/10.1111/1755-0998.12617
372034 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Obtaining fundamental biodiversity metrics such as alpha, beta and gamma
diversity for arthropods is often complicated by a lack of prior taxonomic
information and/or taxonomic expertise, which can result in unreliable
morphologically based estimates. We provide a set of standardized
ecological and molecular sampling protocols that can be employed by
researchers whose taxonomic skills may be limited, and where there may be
a lack of robust a priori information regarding the regional pool of
species. These protocols combine mass sampling of arthropods,
classification of samples into parataxonomic units (PUs) and selective
sampling of individuals for mtDNA sequencing to infer biological species.
We sampled ten lowland rainforest plots located on the volcanic oceanic
island of Réunion (Mascarene archipelago) for spiders, a group with
limited taxonomic and distributional data for this region. We classified
adults and juveniles into PUs and then demonstrated the reconciliation of
these units with presumed biological species using mtDNA sequence data,
ecological data and distributional data. Because our species assignment
protocol is not reliant upon prior taxonomic information, or taxonomic
expertise, it minimizes the problem of the Linnean shortfall to yield
diversity estimates that can be directly compared across independent
studies. Field sampling can be extended to other arthropod groups and
habitats by adapting our field sampling protocol accordingly.
GL alignmentsThis zip file contains DNA sequence alignments for each of
the 57 genetic lineages described in the paper. Sequences headers begin
with the genetic lineage name (e.g. GL01a), then the sampling plot (e.g.
02) and finish with the sample number (e.g. ADN543. Sequence header
information beginning with MF (e.g. MF31) should be ignored.
Réunion