10.5061/DRYAD.8G1NN0J
Saykali, Bechara
Université Libre de Bruxelles
Mathiah, Navrita
Université Libre de Bruxelles
Nahaboo, Wallis
Université Libre de Bruxelles
Racu, Marie-Lucie
Université Libre de Bruxelles
Hammou, Latifa
Université Libre de Bruxelles
Defrance, Matthieu
Université Libre de Bruxelles
Migeotte, Isabelle
Université Libre de Bruxelles
Data from: Distinct mesoderm migration phenotypes in extra-embryonic and
embryonic regions of the early mouse embryo
Dryad
dataset
2019
Gastrulation
mouse
cell shape
Cell migration
2019-04-11T20:25:15Z
2019-04-11T20:25:15Z
en
https://doi.org/10.7554/eLife.42434
5843433 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
In the gastrulation mouse embryo, epiblast cells delaminate at the
primitive streak to form mesoderm and definitive endoderm, through an
epithelial-mesenchymal transition. Mosaic expression of a membrane
reporter in nascent mesoderm enabled recording cell shape and trajectory
through live imaging. Upon leaving the streak, cells changed shape and
extended protrusions of distinct size and abundance depending on the
neighboring germ layer, as well as the region of the embryo. Embryonic
trajectories were meandrous but directional, while extra-embryonic
mesoderm cells showed little net displacement. Embryonic and
extra-embryonic mesoderm transcriptomes highlighted distinct guidance,
cytoskeleton, adhesion, and extracellular matrix signatures. Specifically,
intermediate filaments were highly expressed in extra-embryonic mesoderm,
while live imaging for F-actin showed abundance of actin filaments in
embryonic mesoderm only. Accordingly, RhoA or Rac1 conditional deletion in
mesoderm inhibited embryonic, but not extra-embryonic mesoderm migration.
Overall, this indicates separate cytoskeleton regulation coordinating the
morphology and migration of mesoderm subpopulations.
RNASeq_mouse embryo mesodermThis file contains the following excel files:
Sample list, Expression levels, Ranked list of differential
expressionZip1.zip
Brussels
Belgium