10.5061/DRYAD.8D8V4
McGrath, John S.
Heriot-Watt University
Quist, Jos
University of Twente
Seddon, James R. T.
University of Twente
Lai, Stanley C. S.
University of Twente
Lemay, Serge G.
University of Twente
Bridle, Helen L.
Heriot-Watt University
Data from: Deformability assessment of waterborne protozoa using a
microfluidic-enabled force microscopy probe
Dryad
dataset
2016
2016-03-10T15:37:29Z
2016-03-10T15:37:29Z
en
https://doi.org/10.1371/journal.pone.0150438
422514069 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Many modern filtration technologies are incapable of the complete removal
of Cryptosporidium oocysts from drinking-water. Consequently,
Cryptosporidium-contaminated drinking-water supplies can severely
implicate both water utilities and consumers. Existing methods for the
detection of Cryptosporidium in drinking-water do not discern between
non-pathogenic and pathogenic species, nor between viable and non-viable
oocysts. Using FluidFM, a novel force spectroscopy method employing
microchannelled cantilevers for single-cell level manipulation, we
assessed the size and deformability properties of two species of
Cryptosporidium that pose varying levels of risk to human health. A
comparison of such characteristics demonstrated the ability of FluidFM to
discern between Cryptosporidium muris and Cryptosporidium parvum with 86%
efficiency, whilst using a measurement throughput which exceeded 50
discrete oocysts per hour. In addition, we measured the deformability
properties for untreated and temperature-inactivated oocysts of the highly
infective, human pathogenic C. parvum to assess whether deformability may
be a marker of viability. Our results indicate that untreated and
temperature-inactivated C. parvum oocysts had overlapping but
significantly different deformability distributions.
Cryptosporidium deformability - dataThe files within the ZIP archive are
in CSV format and can be readily accessed by Microsoft Excel, MATLAB, and
many other programs. CSV files were recorded by the CyUI software from
Cytosurge AG (Glattbrug, Switzerland). "spec.fwd.csv" and
"spec.bwd.csv" files refer to the approach or retraction phases
of the force spectroscopy measurements, respectively. Only the
"spec.fwd.csv" (approach) files were used in the data analysis.
Cantilever spring constants, deflection sensitivities, and sampling
frequencies are specified in the header of the csv files. The initial
cantilever-substrate separation was 10 micron for the C. muris and 5.0-5.3
micron for the C. parvum measurements.