10.5061/DRYAD.87V3V
Piaggio, Antoinette J.
United States Department of Agriculture
Engeman, Richard M.
United States Department of Agriculture
Hopken, Matthew W.
United States Department of Agriculture
Humphrey, John S.
United States Department of Agriculture
Keacher, Kandy L.
United States Department of Agriculture
Bruce, William E.
United States Department of Agriculture
Avery, Michael L.
United States Department of Agriculture
Data from: Detecting an elusive invasive species: a diagnostic PCR to
detect Burmese python in Florida waters and an assessment of persistence
of environmental DNA
Dryad
dataset
2013
Python bivitattus
reptile
Burmese python
DNA persistence
2013-10-04T18:39:16Z
2013-10-04T18:39:16Z
en
https://doi.org/10.1111/1755-0998.12180
381106 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Recent studies have demonstrated that detection of environmental DNA
(eDNA) from aquatic vertebrates in water bodies is possible. The Burmese
python, Python bivittatus, is a semi-aquatic, invasive species in Florida
where its elusive nature and cryptic coloration make its detection
difficult. Our goal was to develop a diagnostic PCR to detect P.
bivittatus from water-borne eDNA, which could assist managers in
monitoring this invasive species. First, we used captive P. bivittatus to
determine whether reptilian DNA could be isolated and amplified from water
samples. We also evaluated the efficacy of two DNA isolation methods and
two DNA extraction kits commonly used in eDNA preparation. A fragment of
the mitochondrial cytochrome b gene from P. bivittatus was detected in all
water samples isolated with the sodium acetate precipitate and the QIAamp
DNA Micro Kit. Next, we designed P. bivittatus-specific primers and
assessed the degradation rate of eDNA in water. Our primers did not
amplify DNA from closely related species, and we found that P. bivittatus
DNA was consistently detectable up to 96 h. Finally, we sampled water from
six field sites in south Florida. Samples from five sites, where P.
bivittatus has been observed, tested positive for eDNA. The final site was
negative and had no prior documented evidence of P. bivittatus. This study
shows P. bivittatus eDNA can be isolated from water samples; thus, this
method is a new and promising technique for the management of invasive
reptiles.
ABI Fragment analysis PybiCB3 Pentest II 6-18-13_15 minutes onlyABI
fragment analysis screen shot to show peaks generated when using the
primers we developed in Pen test II plus flourescent labels.Gel PybiCB3
Pentest II 6-18-13_15 minutes onlyPicture of bands generated by running
PCR product using the primers we dveloped in Pen test II (unlabeled) on
agarose gel.PybiCB3 primers full sequenceFASTA file showing sequence
produced from PCR product using primers we develeoped in Pen Test
IIQA-1950 Python all results 6-25-2013