10.5061/DRYAD.864RV
Bradbury, Ian R.
Dalhousie University
Fisheries and Oceans Canada
Wringe, Brendan F.
Fisheries and Oceans Canada
Watson, Beth
Dalhousie University
Paterson, Ian
Dalhousie University
Horne, John
Dalhousie University
Beiko, Robert
Dalhousie University
Lehnert, Sarah J.
Fisheries and Oceans Canada
Clément, Marie
Memorial University of Newfoundland
Marine Institute
Anderson, Eric C.
Southwest Fisheries Science Center
Jeffery, Nicholas W.
Fisheries and Oceans Canada
Duffy, Steven
Fisheries and Oceans Canada
Sylvester, Emma
Dalhousie University
Fisheries and Oceans Canada
Robertson, Martha
Fisheries and Oceans Canada
Bentzen, Paul
Dalhousie University
Data from: Genotyping-by-sequencing of genome-wide microsatellite loci
reveals fine-scale harvest composition in a coastal Atlantic salmon
fishery
Dryad
dataset
2018
Mixed-stock analysis
Salmo salar
Next-generation sequencing
Genetic assignment
2018-01-29T16:46:43Z
2018-01-29T16:46:43Z
en
https://doi.org/10.1111/eva.12606
772978 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Individual assignment and genetic mixture analysis are commonly utilized
in contemporary wildlife and fisheries management. Although microsatellite
loci provide unparalleled numbers of alleles per locus, their use in
assignment applications is increasingly limited. However, next-generation
sequencing, in conjunction with novel bioinformatic tools allows large
numbers of microsatellite loci to be simultaneously genotyped, presenting
new opportunities for individual assignment and genetic mixture analysis.
Here we scanned the published Atlantic salmon genome to identify 706
microsatellite loci, from which we developed a final panel of 101
microsatellites distributed across the genome (average 3.4 loci per
chromosome). Using samples from 35 Atlantic salmon populations (n=1485
individuals) from coastal Labrador, Canada, a region characterized by low
levels of differentiation in this species, this panel identified 844
alleles (average of 8.4 alleles per locus). Simulation-based evaluations
of assignment and mixture identification accuracy revealed unprecedented
resolution, clearly identifying 26 rivers or groups of rivers spanning 500
km of coastline. This baseline was used to examine the stock composition
of 696 individuals harvested in the Labrador Atlantic salmon fishery and
revealed that coastal fisheries largely targeted regional groups
(<300km). This work suggests that the development and application
of large sequenced microsatellite panels presents great potential for
stock resolution in Atlantic salmon and more broadly in other exploited
anadromous and marine species.
Labrador Atlantic Salmon Sequenced Microsatellite DataGenepop file of
sequenced microsatellite genotype data for 35 salmon populations in
Labrador Canada.
Canada
Labrador