10.5061/DRYAD.7S8H7
Delroisse, Jérôme
University of Mons
Ullrich-Lüter, Esther
Museum für Naturkunde
Ortega-Martinez, Olga
University of Gothenburg
Dupont, Sam
University of Gothenburg
Arnone, Maria-Ina
Stazione Zoologica Anton Dohrn
Mallefet, Jérôme
Université Catholique de Louvain
Flammang, Patrick
University of Mons
Data from: High opsin diversity in a non-visual infaunal brittlestar
Dryad
dataset
2015
Amphiura filiformis
Echinodermata
photoreception
Ophiuroidea
2015-11-14T00:00:00Z
2015-11-14T00:00:00Z
en
https://doi.org/10.1186/1471-2164-15-1035
75668 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Background: In metazoans, opsins are photosensitive proteins involved in
both vision and non-visual photoreception. Echinoderms have no
well-defined eyes but several opsin genes were found in the purple sea
urchin (Strongylocentrotus purpuratus) genome. Molecular data are lacking
for other echinoderm classes although many species are known to be light
sensitive.Results: In this study focused on the European brittle star
Amphiura filiformis, we first highlighted a blue-green light sensitivity
using a behavioural approach. We then identified 13 new putative opsin
genes against eight bona fide opsin genes in the genome of S. purpuratus.
Six opsins were included in the rhabdomeric opsin group (r-opsins). In
addition, one putative ciliary opsin (c-opsin), showing high similarity
with the c-opsin of S. purpuratus (Sp-opsin 1), one Go opsin similar to
Sp-opsins 3.1 and 3.2, two basal-branch opsins similar to Sp-opsins 2 and
5, and two neuropsins similar to Sp-opsin 8, were identified. Finally, two
sequences from one putative RGR opsin similar to Sp-opsin 7 were also
detected. Adult arm transcriptome analysis pinpointed opsin mRNAs
corresponding to one r-opsin, one neuropsin and the homologue of Sp-opsin
2. Opsin phylogeny was determined by maximum likelihood and Bayesian
analyses. Using antibodies designed against c- and r-opsins from S.
purpuratus, we detected putative photoreceptor cells mainly in spines and
tube feet of A. filiformis, respectively. The r-opsin expression pattern
is similar to the one reported in S. purpuratus with cells labelled at the
tip and at the base of the tube feet. In addition, r-opsin positive cells
were also identified in the radial nerve of the arm. C-opsins positive
cells, expressed in pedicellariae, spines, tube feet and epidermis in S.
purpuratus were observed at the level of the spine stroma in the brittle
star. Conclusion: Light perception in A. filiformis seems to be mediated
by opsins (c- and r-) in, at least, spines, tube feet and in the radial
nerve cord. Other non-visual opsin types could participate to the light
perception process indicating a complex expression pattern of opsins in
this infaunal brittle star.
Truncated Opsin Alignment (including New Amphiura opsins)Fasta file of the
truncated opsin alignment used for phylogenetic
analyses.Delroisse_et_al_2014_Truncated_Alignment_Opsin_AmphiuraFASTABayesian tree of new Amphiura opsinsTree files obtained in Bayesian analyses (modified with FigTree software, http://tree.bio.ed.ac.uk/software/figtree/)Bayesian_tree_Delroisse_et_al_2014_Opsin_Amphiura.treeML tree of new Amphiura opsinsTREE files obtained in Maximum Likelihood Analyses (modified with FigTree software, http://tree.bio.ed.ac.uk/software/figtree/)ML_tree_Delroisse_et_al_2014_Opsin_Amphiura.tree
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