10.5061/DRYAD.70254
Keller, Stephen R.
University of Maryland, College Park
Trott, Regina
University of Maryland, College Park
Pylant, Cortney
University of Maryland, College Park
Nelson, David N.
University of Maryland, College Park
Santos, Scott R.
Auburn University
Data from: "Genome-wide microsatellite marker development from
next-generation sequencing of two non-model bat species impacted by wind
turbine mortality: Lasiurus borealis and L. cinereus
(Vespertilionidae)" in Genomic Resources Notes accepted 1 October
2013 to 30 November 2013
Dryad
dataset
2014
Habitat Degradation
Lasiurus borealis
Wildlife Management
Lasiurus cinereus
2014-01-14T18:10:00Z
2014-01-14T18:10:00Z
en
https://doi.org/10.1111/1755-0998.12221
6671835 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Tree-roosting bats in the genus Lasiurus are widespread, migratory species
that have not been well characterized for population genetic diversity and
structure due to a lack of genetic resources. Generating genetic resources
in Lasiurus is made pressing by the need for conservation genetic
assessments of demographic trends in this genus, which comprise a large
percentage of bat mortalities at wind turbine sites across North America.
We report on marker development from whole-genome Illumina sequencing of
the red bat (Lasirus borealis) and the hoary bat (L. cinereus). We
generated paired-end libraries for a single individual of each species,
sequenced on the Illumina HiSeq platform. We mapped a total of 46.6
million reads to the Myotis lucifigus reference genome, and used
bioinformatics searches to identify tends of thousands of simple sequence
repeats (SSRs) distributed across the bat genome. We selected 48 candidate
microsatellite loci to develop cross-species primer sequences for
Lasiurus, assembled these into multiplex combinations, and tested for
amplification and polymorphism levels in a sample of 23 individuals from
each of L. borealis and L. cinereus. In total, we identified 42 highly
polymorphic loci that could be robustly amplified and scored, the majority
of which (39) were also combinable into highly multiplexed assays of 4-8
loci each. The combination of new genomic sequence assemblies, a large set
of highly polymorphic microsatellite loci, and the ability to efficiently
multiplex represents a significant contribution to the genetic resources
available for population and comparative genetic studies of bats.
Keller_etal_2013MER_MSATCOMMANDER_microsatsMicrosoft Excel file containing
the results of the MSATCOMMANDER searches for simple sequence repeats
(SSR's) in the genomic sequence of two bat species: Lasirus borealis
and L. cinereus
(Vespertilionidae).Keller_etal_2013MER_MSATCOMMANDER_primersMicrosoft
Excel file of primers developed by MSATCOMMANDER for SSR loci from genomic
sequencing of two bats species: Lasirus borealis and L.
cinereus.Keller_etal_MER2013_Lasiurus_pipelineUNIX bash script containing
the informatics pipeline used to align sequence reads for Lasiurus
borealis and L. cinereus against the reference genome and determine
consensus sequences.
eastern North America