10.5061/DRYAD.6FD1N
Geiger, Matthias F.
Zoological Research Museum Alexander Koenig
Herder, Fabian
National Museum of Natural History; José Gutiérrez Abascal 2 28006 Madrid Spain
Monaghan, Michael T.
Leibniz Institute of Freshwater Ecology and Inland Fisheries
Almada, Vitor
Instituto Universitário de Ciências Psicológicas, Sociais e da Vida
Barbieri, Roberta
Hellenic Centre for Marine Research
Bariche, Michel
American University of Beirut
Berrebi, Patrick
Institut des Sciences de l'Evolution de Montpellier
Bohlen, Jörg
Institute of Animal Physiology and Genetics
Casal-Lopez, Miriam
National Museum of Natural History
Denys, Gaël P. J.
Zoological Research Museum Alexander Koenig
Dettai, Agnès
Institute of Animal Physiology and Genetics
Doadrio, Ignacio
National Museum of Natural History
Kalogianni, Elena
Hellenic Centre for Marine Research
Kärst, Heiko
Deutsche Killifischgemeinschaft e.V.; 65183 Wiesbaden Germany
Kottelat, Maurice
Route de la Baroche; 2952 Cornol Switzerland
Kovačić, Marcelo
University of Zagreb
Laporte, Martin
University of Perugia
Lorenzoni, Massimo
University of Perugia
Marčić, Zoran
University of Zagreb
Özuluğ, Müfit
Istanbul University
Perdices, Anabel
National Museum of Natural History
Perea, Silvia
National Museum of Natural History
Persat, Henri
Claude Bernard University Lyon 1
Porcellotti, Stefano
Associazione Ichthyos Italia; Via. A Cecchi 12 52100 Arezzo Italy
Puzzi, Cesare
Gestione e Ricerca Ambientale Ittica Acque; 21020 Varano Borghi Italy
Robalo, Joana
Hellenic Centre for Marine Research
Šanda, Radek
National Museum
Schneider, Michael
Deutsche Killifischgemeinschaft e.V.; 65183 Wiesbaden Germany
Šlechtová, Vendula
Institute of Animal Physiology and Genetics
Stoumboudi, M.
Hellenic Centre for Marine Research
Walter, Simon
Zoological Research Museum Alexander Koenig
Freyhof, Joerg
Leibniz Institute of Freshwater Ecology and Inland Fisheries
Delmastro, G. B.
Natural History Museum
Natural History Museum
Data from: Spatial heterogeneity in the Mediterranean Biodiversity Hotspot
affects barcoding accuracy of its freshwater fishes
Dryad
dataset
2014
Wildlife Management
2014-03-27T18:14:54Z
2014-03-27T18:14:54Z
en
https://doi.org/10.1111/1755-0998.12257
2245404 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Incomplete knowledge of biodiversity remains a stumbling block for
conservation planning, and even occurs within globally important
Biodiversity Hotspots. Although technical advances have boosted the power
of molecular biodiversity assessments, the link between DNA sequences and
species and the analytics to discriminate entities, remain crucial. Here,
we present an analysis of the first DNA barcode library for the freshwater
fish fauna of the Mediterranean Biodiversity Hotspot (526 spp.), with
virtually complete species coverage (498 spp., 98% extant species). In
order to build an identification system supporting conservation, we
compared species determination by taxonomists to multiple clustering
analyses of DNA barcodes for 3165 specimens. The congruence of barcode
clusters with morphological determination was strongly dependent on the
method of cluster delineation, but was highest with the GMYC model-based
approach (83% of all species recovered as GMYC entity). Overall,
genetic-morphological discontinuities suggest the existence of up to 64
previously unrecognized candidate species. We found reduced identification
accuracy when using the entire DNA-barcode database, compared to analyses
on databases for individual river catchments. This scale effect has
important implications for barcoding assessments, and suggests that fairly
simple identification pipelines provide sufficient resolution in local
applications. We calculated EDGE (Evolutionarily Distinct and Globally
Endangered) scores in order to identify candidate species for conservation
priority, and argue that the evolutionary content of barcode data can be
used to detect priority species for future IUCN assessments. We show that
large-scale barcoding inventories of complex biotas are feasible and
contribute directly to the evaluation of conservation priorities.
COI sequence alignmentDNA sequence alignment containing all 3165
individuals. Sequence labels contain genus, species, drainage system,
country of origin, field or museum ID, and sample ID.3165dryad.fasta