10.5061/DRYAD.6D3V0
Jensen, Lindy M
Smith College
Grant, Jessica R
Smith College
Laughinghouse IV, H Dail
Smith College
Katz, Laura A.
University of Massachusetts Amherst
Jensen, Lindy
University of Michigan-Ann Arbor
Data from: Assessing the effects of a sequestered germline on interdomain
lateral gene transfer in Metazoa
Dryad
dataset
2016
gene loss
lateral gene transfer
Animals
Archaea
weak link model
2016-05-06T16:34:15Z
2016-05-06T16:34:15Z
en
https://doi.org/10.1111/evo.12935
337863 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
A sequestered germline in Metazoa has been argued to be an obstacle to
lateral gene transfer (LGT), though few studies have specifically assessed
this claim. Here we test the hypothesis that the origin of a sequestered
germline reduced LGT events in Bilateria (i.e. triploblast lineages) as
compared to early-diverging Metazoa (i.e. Ctenophora, Cnidaria, Porifera,
and Placozoa). We analyze single-gene phylogenies generated with over 900
species, sampled from among Bacteria, Archaea and Eukaryota to identify
well-supported interdomain LGTs. We focus on interdomain LGT (i.e. those
between prokaryotes and Metazoa) as systematic errors in single gene tree
reconstruction create uncertainties for interpreting
eukaryote-to-eukaryotes transfers. The breadth of the sampled Metazoa
enables us to estimate the timing of LGTs, and to examine the pattern
before vs. after the evolution of a sequestered germline. We identified 58
LGTs found only in Metazoa and prokaryotes (i.e. bacteria and/or archaea),
and 7 genes transferred from prokaryotes into Metazoa plus one other
eukaryotic clade. Our analyses indicate that more interdomain transfers
occurred before the development of a sequestered germline, consistent with
the hypothesis that this feature is an obstacle to LGT.
OG# to Protein ListAn excel file listing the protein name, abbreviation,
found KEGG function (if known), and the corresponding OG5 number of the
alignment for all 58 presence/absence lateral gene transfers found. Each
animo acid sequence alignment file is labeled with a unique
OG5#.OG_to_Protein_List.xlsxTopologies with two LGT EventsSingle gene
trees in Newick format for the seven genes found with topologies
consistent with two lateral gene
transfers.Double_event_topologies.zipPresence/absence alignmentsAll 58
laterally transferred presence/absence animo acid sequence alignments
found. Files are in text format.Presence_absence_alignments.zip