10.5061/DRYAD.606J8
Wybouw, Nicky
University of Amsterdam
Zhurov, Vladimir
Western University
Martel, Catherine
Western University
Bruinsma, Kristie A.
Western University
Hendrickx, Frederik
Royal Belgian Institute of Natural Sciences
Ghent University
Grbić, Vojislava
Western University
Van Leeuwen, Thomas
University of Amsterdam
Ghent University
Data from: Adaptation of a polyphagous herbivore to a novel host plant
extensively shapes the transcriptome of herbivore and host
Dryad
dataset
2015
Xenobiotic metabolism
genetic accommodation
2015-07-22T13:27:55Z
2015-07-22T13:27:55Z
en
https://doi.org/10.1111/mec.13330
9801530 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Generalist arthropod herbivores rapidly adapt to a broad range of host
plants. However, the extent of transcriptional reprogramming in the
herbivore and its hosts associated with adaptation remains poorly
understood. Using the spider mite Tetranychus urticae and tomato as models
with available genomic resources, we investigated the reciprocal
genomewide transcriptional changes in both spider mite and tomato as a
consequence of mite's adaptation to tomato. We transferred a
genetically diverse mite population from bean to tomato where triplicated
populations were allowed to propagate for 30 generations. Evolving
populations greatly increased their reproductive performance on tomato
relative to their progenitors when reared under identical conditions,
indicative of genetic adaptation. Analysis of transcriptional changes
associated with mite adaptation to tomato revealed two main components.
First, adaptation resulted in a set of mite genes that were constitutively
downregulated, independently of the host. These genes were mostly of an
unknown function. Second, adapted mites mounted an altered transcriptional
response that had greater amplitude of changes when re-exposed to tomato,
relative to nonadapted mites. This gene set was enriched in genes encoding
detoxifying enzymes and xenobiotic transporters. Besides the direct
effects on mite gene expression, adaptation also indirectly affected the
tomato transcriptional responses, which were attenuated upon feeding of
adapted mites, relative to the induced responses by nonadapted mite
feeding. Thus, constitutive downregulation and increased transcriptional
plasticity of genes in a herbivore may play a central role in adaptation
to host plants, leading to both a higher detoxification potential and
reduced production of plant defence compounds.
Spider mite performance on different host plants, before and after tomato
adaptation.Performance was quantified as the total population sizes
(including eggs and mobile stages) 10 days after the initial inoculation
using 35 female mites. The T. urticae London mite strain and the following
plant cultivars were used; bean: Phaseolus vulgaris L. cv ‘Prelude’,
cucumber: Cucumis sativus L. cv ‘Tanja’, tomato: Solanum lycopersicum L.
cv ‘Moneymaker’ and bell pepper: Capsicum annuum L. cv ‘California
Wonder’.T-urticae_performance_host-plants.txtTomato foliar damage by
spider mite feedingFoliar damage was measured using chlorotic spots as
described in Kant et al.
(2004).T-urticae_tomato_leaf_damage.txtDifferentially expressed genes of
T. urticae in the experimental evolutionary set-upDifferent DEG lists,
arising from different transcriptomic comparisons, are shown in different
tabs of the excel file. The abbreviations are explained in Table 1 of the
main text.T-urticae_differential_gene-expression.xlsxDifferentially
expressed genes of tomato in the experimental evolutionary set-upThe
abbreviations are explained in Table 1 of the main
text.Tomato_differential_gene-expression.xlsAnnotations of T. urticae
genesBLAST2GO- and best-BLAST-hit-annotations are included. T. urticae
gene IDs can be accessed at
http://bioinformatics.psb.ugent.be/orcae/overview/Teturmite_annotation-descriptions.txtAnnotations of tomato genesITAG, Blast2GO and EC descriptions for tomato genes are included.tomato_annotation-descriptions.txt