10.5061/DRYAD.5Q91D
Vallejo-Marin, Mario
University of Stirling
Cooley, Arielle M.
Whitman College
Lee, Michelle Yuequi
University of Stirling
Folmer, Madison
College of William & Mary
McKain, Michael R.
Donald Danforth Plant Science Center
Puzey, Joshua Robert
College of William & Mary
Data from: Strongly asymmetric hybridization barriers shape the origin of
a new polyploid species and its hybrid ancestor
Dryad
dataset
2016
mitochondrial genome
allopolyploidy
Asexual reproduction
Mimulus luteus
Mimulus peregrinus
sterile hybrid
triploid block
Mimulus robertsii
introduced species
Mimulus guttatus
genome skimming
2016-06-30T05:31:35Z
2016-06-30T05:31:35Z
en
https://doi.org/10.3732/ajb.1500471
181394432 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
PREMISE OF THE STUDY: Hybridization between diploids and tetraploids can
lead to new allopolyploid species, often via a triploid intermediate.
Viable triploids are often produced asymmetrically, with greater success
observed for “maternal-excess” crosses where the mother has a higher
ploidy than the father. Here we investigated the evolutionary origins of
Mimulus peregrinus, an allohexaploid recently derived from the triploid M.
×robertsii, to determine whether reproductive asymmetry has shaped the
formation of this new species. METHODS: We used reciprocal crosses between
the diploid (M. guttatus) and tetraploid (M. luteus) progenitors to
determine the viability of triploid M. ×robertsii hybrids resulting from
paternal- vs. maternal-excess crosses. To investigate whether experimental
results predict patterns seen in the field, we performed parentage
analyses comparing natural populations of M. peregrinus to its diploid,
tetraploid, and triploid progenitors. Organellar sequences obtained from
pre-existing genomic data, supplemented with additional genotyping was
used to establish the maternal ancestry of multiple M. peregrinus and M.
×robertsii populations. KEY RESULTS: We found strong evidence for
asymmetric origins of M. peregrinus, but opposite to the common pattern,
with paternal-excess crosses significantly more successful than
maternal-excess crosses. These results successfully predicted hybrid
formation in nature: 111 of 114 M. ×robertsii individuals, and 27 of 27 M.
peregrinus, had an M. guttatus maternal haplotype. CONCLUSION: This study,
which includes the first Mimulus chloroplast genome assembly, demonstrates
the utility of parentage analysis through genome skimming. We highlight
the benefits of complementing genomic analyses with experimental
approaches to understand asymmetry in allopolyploid speciation.
Phylogenetic Analyses (trees, alignments, filtered regions)This zipped
directory contains the phylogenetic trees, sequence alignments, and
filtered regions.PhylogeneticAnalayses.tar
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