10.5061/DRYAD.5DV41NS7Z
Flomm, Felix
Heinrich-Pette-Institute
Soh, Timothy K
Heinrich-Pette-Institute
Schneider, Carola
Heinrich-Pette-Institute
Wedemann, Linda
Hannover Medical School
Britt, Hannah M
0000-0001-8510-0331
University College London
Thalassinos, Konstantinos
University College London
Pfitzner, Soeren
0000-0002-0155-0317
Heinrich-Pette-Institute
Reimer, Rudolph
Heinrich-Pette-Institute
Grünewald, Kay
Heinrich-Pette-Institute
Bosse, Jens Bernhard
0000-0001-7252-5541
Hannover Medical School
Correlative 3D SBFSEM data from: Intermittent bulk release of human
cytomegalovirus
Dryad
dataset
2022
FOS: Biological sciences
Assembly
Egress
CLEM
Serial Block-Face Scanning Electron Microscopy (SBFSEM)
HCMV
Human cytomegalovirus
Microscopy
Wellcome Trust
https://ror.org/029chgv08
209250/Z/17/Z
Deutsche Forschungsgemeinschaft
https://ror.org/018mejw64
390874280
Deutsche Forschungsgemeinschaft
https://ror.org/018mejw64
413831413
German National Academic Foundation
https://ror.org/05xwwfy96
Deutsche Forschungsgemeinschaft
https://ror.org/018mejw64
BO 4158/5-1
Freie und Hansestadt Hamburg*
LFF-FV 71-2019
Leibniz Association
https://ror.org/01n6r0e97
W6/2018
Wellcome Trust
https://ror.org/029chgv08
104913/Z/14/Z
Centre for Structural Systems Biology
2022-07-19T00:00:00Z
2022-07-19T00:00:00Z
en
https://doi.org/10.1371/journal.ppat.1010575
https://doi.org/10.5061/dryad.gtht76hpt
https://doi.org/10.5061/dryad.jq2bvq8bw
120633440181 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Human Cytomegalovirus (HCMV) can infect a variety of cell types by using
virions of varying glycoprotein compositions. It is still unclear how this
diversity is generated, but spatio-temporally separated envelopment and
egress pathways might play a role. So far, one egress pathway has been
described in which HCMV particles are individually enveloped into small
vesicles and are subsequently exocytosed continuously. However, some
studies have also found enveloped virus particles inside multivesicular
structures but could not link them to productive egress or degradation
pathways.We used a novel 3D-CLEM workflow allowing us to investigate these
structures in HCMV morphogenesis and egress at high spatio-temporal
resolution. We found that multiple envelopment events occurred at
individual vesicles leading to multiviral bodies (MViBs), which
subsequently traversed the cytoplasm to release virions as intermittent
bulk pulses at the plasma membrane to form extracellular virus
accumulations (EVAs). Our data support the existence of a novel bona fide
HCMV egress pathway, which opens the gate to evaluate divergent egress
pathways in generating virion diversity.
Details on data acquisition and processing can be found in the original
publication. The uploaded here are the unprocessed raw data.
The data are in the proprietary dm4 format as saved by the acquisition
software. However, they can be easily opened and converted in Fiji/ImageJ
with the Bioformats importer. We recommend alignment of the stack and
binning of 2x2 for working with the data.