10.5061/DRYAD.5C960
Villacorta-Rath, Cecilia
University of Tasmania
Ilyushkina, Irina
Victoria University of Wellington
Strugnell, Jan M.
La Trobe University
Green, Bridget S.
University of Tasmania
Murphy, Nicholas P.
La Trobe University
Doyle, Stephen R.
La Trobe University
Hall, Nathan E.
La Trobe University
Robinson, Andrew J.
La Trobe University
Bell, James J.
Victoria University of Wellington
Data from: Outlier SNPs enable food traceability of the southern rock
lobster, Jasus edwardsii
Dryad
dataset
2017
Jasus edwardsii
lobster
food traceability
2017-09-14T00:00:00Z
2017-09-14T00:00:00Z
en
https://doi.org/10.1007/s00227-016-3000-1
56938 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Recent advances in next-generation sequencing have enhanced the resolution
of population genetic studies of non-model organisms through increased
marker generation and sample throughput. Using double digest restriction
site-associated DNA sequencing (ddRADseq), we investigated the population
structure of the commercially important southern rock lobster, Jasus
edwardsii, in Australia and New Zealand with the aim of identifying a
panel of SNP markers that could be used to trace country of origin. Four
ddRADseq libraries comprising a total of 88 individuals were sequenced on
the Illumina MiSeq platform, and demultiplexed reads were used to create a
reference catalog of loci. Individual reads were then mapped to the
reference catalog, and variant calling was performed. We have
characterized two single-nucleotide polymorphism (SNP) panels comprised in
total of 656 SNPs. The first panel contained 535 neutral SNPs and the
second, 121 outlier SNPs that were characteristic of being putatively
under selection. Both neutral and outlier SNP panels showed significant
differentiation between the two countries, with the outlier loci
demonstrating much larger FST values (FST outlier SNP panel = 0.134, P
< 0.0001; FST neutral SNP panel = 0.022, P < 0.0001).
Assignment tests performed with the outlier SNP panel allocated 100 % of
the individuals to country of origin, demonstrating the usefulness of
these markers for food traceability of J. edwardsii.
Neutral-lociFasta file containing sequences of 535 putatively neutral
lociOutlier-lociFasta file containing sequences of 121 loci putatively
under selection
New Zealand
Australia