10.5061/DRYAD.588D7
Bartkowska, Magdalena P.
Queen's University
Wong, Andy Y-C
Queen's University
Sagar, Stephen P.
Queen's University
Zeng, Lily
Queen's University
Eckert, Christopher G.
Data from: Lack of spatial structure for phenotypic and genetic variation
despite high self-fertilization in Aquilegia canadensis (Ranunculaceae)
Dryad
dataset
2018
analysis of molecular variance
Aquilegia canadensis
hierarchical genetic structure
Bayesian cluster analysis
floral morphology
Holocene
2018-01-30T15:31:13Z
2018-01-30T15:31:13Z
en
https://doi.org/10.1038/s41437-018-0065-2
57608 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
By reducing genetically effective population size and gene flow,
self-fertilization should lead to strong spatial genetic structure (SGS).
Although the short-lived plant Aquilegia canadensis produces large,
complex, nectar-rich flowers, 75% of seed, on average, are
self-fertilized. Previous experimental results are consistent with the
fine-scale SGS expected in selfing populations. In contrast, key floral
traits show no evidence of SGS, despite a significant genetic basis to
phenotypic variation within populations. In this study, we attempt to
resolve these contradictory results by hierarchically sampling plants from
two plots nested within each of seven rock outcrops distributed over
several km, and comparing the spatial pattern of phenotypic variation in
four floral traits with neutral genetic variation at 10 microsatellite
loci. For both floral and microsatellite variation, we detected only weak
hierarchical structuring and no isolation by distance. The spatial pattern
of variation in floral traits was on par with microsatellite
polymorphisms. These results suggest regular long-distance gene flow via
pollen. At much finer spatial scales within plots, estimates of
relatedness were higher (albeit very low) between nearest neighbours than
random plants, and declined with increasing distance between neighbours,
consistent with highly localized seed dispersal. High selfing should yield
SGS, but strong inbreeding depression in A. canadensis likely erodes SGS
so that reproductive plants exhibit weak structure typical of outcrossers,
especially given that outcrossing and consequent gene flow in this species
are mediated by strong-flying hummingbirds and bumble bees.
HierstructWe present morphological and genotypic observations for plants
observed in the field. Individuals were sampled hierarchically from two
plots nested within each of seven rock outcrops distributed over several
km. Phenotypic variation in four floral traits was compared with neutral
genetic variation at microsatellite loci (9 loci used for AMOVA type
analyses and 10 loci used for nearest-neighbour analysis). Six data files
are contained within this project.
Ontario Canada