10.5061/DRYAD.57SG5
Brunet, Frédéric G.
Claude Bernard University Lyon 1
Fraser, Fiona W.
Deakin University
Binder, Marley J.
Deakin University
Smith, Adam D.
Deakin University
Kintakas, Christopher
Deakin University
Dancevic, Carolyn M.
Deakin University
Ward, Alister C.
Deakin University
McCulloch, Daniel R.
Deakin University
Data from: The evolutionary conservation of the A Disintegrin-like and
Metalloproteinase domain with Thrombospondin-1 motif metzincins across
vertebrate species and their expression in teleost zebrafish
Dryad
dataset
2015
Whole Genome Duplications
Vertebrata
ADAMTS
2015-03-04T19:43:01Z
2015-03-04T19:43:01Z
en
https://doi.org/10.1186/s12862-015-0281-9
162137 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Background: The A Disintegrin-like and Metalloproteinase domain with
Thrombospondin-1 motifs (ADAMTS) enzymes comprise 19 mammalian
zinc-dependent metalloproteinases (metzincins) with homologues in a wide
range of invertebrates. ADAMTS enzymes have a broad range of functions in
development and diseases due to their extracellular matrix remodelling
activity. Here, we report a detailed characterisation of their
evolutionary conservation across vertebrates. Results: Using
bioinformatics complemented with de novo sequencing, gene sequences for
ADAMTS enzymes were obtained from a variety of organisms. Detailed
evolutionary analyses revealed a high level of conservation across
vertebrates with evidence of ADAMTS gene expansion during two rounds of
whole genome duplication (WGD) in vertebrates, while tandem duplication
events and gene loss were also apparent. However, the additional round of
teleost-specific WGD did not have a significant effect on ADAMTS gene
family members suggesting their conserved roles have remained constant in
teleost fish. Quantitative reverse-transcriptase polymerase chain reaction
analysis revealed dynamic expression of adamts genes throughout zebrafish
embryonic development reflecting the key conserved roles they play in
vertebrate embryogenesis. Notably, several adamts mRNAs were maternally
expressed with a dramatic increase in mRNA levels coinciding with zygotic
expression and organogenesis. Broad adamts mRNA expression was also
demonstrated in several adult organs indicating potential roles in adult
homeostasis. Conclusions: Our data highlight the evolution of the ADAMTS
gene family through duplication processes across metazoans supplemented by
a burst of amplification through vertebrate WGD events. It also strongly
posits the zebrafish as a potential model species to further elucidate the
function of ADAMTS enzymes during vertebrate development.
ComplData__Brunet_etal_BMC_6624500221359074.tarSequence alignment data for
ADAMTS proteins across species.