10.5061/DRYAD.5147G9S
Rodrigues, Ernani V.
University of Sao Paulo
Riguete, Júlia R.
Graduate Program in Ecosystem EcologyUniversity Vila Velha Vila Velha ES Brazil
Pereira, Henrique R.C.
Universidade Federal do ABC
Tesch, Juliétty A.
Graduate Program in Ecosystem EcologyUniversity Vila Velha Vila Velha ES Brazil
Silva, Ary G.
Graduate Program in Ecosystem EcologyUniversity Vila Velha Vila Velha ES Brazil
Data from: An affordable apparatus for fine‐controlled emulation of
buzzing frequencies of bees for the testing hypothesis in buzz
interactions
Dryad
dataset
2019
pollination biology
foraging behavior
buzz-pollination
pollen harvesting
2019-05-31T00:00:00Z
2019-05-31T00:00:00Z
en
https://doi.org/10.1002/ece3.4290
14871648 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
1. The buzzing foraging behavior of female bees for pollen harvesting
called the attention of early pollination biologists. Flower-types that
demand this buzzing behavior comprise about 20.000 species of different
and phylogenetically unrelated plant taxa, suggesting that it had
independently evolved many times among the flowering plants. Between the
late 70’s and early 80’s, theoretical papers had modeled the energetics of
buzz-pollination, but, up to this moment, no hypothesis was experimentally
tested concerning the theoretical basis of the energetics of
buzz-pollination. 2. We present a cost-effective and simple apparatus,
including a digital and highly accurate frequency generator, and a device
for the transference of buzz-frequency energy to the receptive floral
unity. The receptive floral unities may comprise the entire or partial
androecium, or the tubular corolla, or, in some cases, the whole flower.
3. This apparatus can be easily used both laboratory and field conditions
of research, since natural air currents are avoided, and the response of
pollen liberation can be quantitatively measured by pollen grain counts
that can be captured by adhesion in slide poured with an isosmotic
lactate-glycerin media. 4. The maximum displacement of the hardwire
beam/claw system was 0.1170 ± 0.0006mm @ 150Hz; 0.021 ± 0.003 mm @ 250 Hz;
0.010 ± 0.001 mm @350 Hz; 0.0058 ± 0.0001 mm @ 450 Hz and 0.0082 ± 0.0005
mm @ 55 0Hz. 5. Hypothesis contrasting frequency emission and pollen
liberation measured as pollen-grain counts may be tested in a species
flower-type by simple linear regression, if pollen counts are normally
distributed, or ordinal logistic regression, with non-normal counts. The
comparison among different flower-type requirements can be tested through
appropriate statistical methods for both normally and non-normally
distributed pollen grain counts.
data_f_150Displacement measured at 150 Hzdata_f_250Displacement at 250
Hzdata_f_350Displacement at 350 Hzdata_f_450Displacement at 450
Hzdata_f_550Displacement at 550 Hz