10.5061/DRYAD.4TMPG4F8C
Park, Chanhyun
0000-0002-0200-6903
University of Tokyo
Sakurai, Yuki
University of Tokyo
Sato, Hirofumi
University of Tokyo
Kanda, Shinji
University of Tokyo
Iino, Yuichi
University of Tokyo
Kunitomo, Hirofumi
0000-0001-7312-7051
University of Tokyo
Roles of the ClC chloride channel CLH-1 in food-associated salt chemotaxis
behavior of C. elegans
Dryad
dataset
2020
FOS: Biological sciences
Japan Society for the Promotion of Science
https://ror.org/00hhkn466
Grant-in-Aid for Scientific Research (S) JP17H06113
Japan Science and Technology Agency
https://ror.org/00097mb19
CREST JP17H06113
Japan Society for the Promotion of Science
https://ror.org/00hhkn466
Grants-in-Aid for Innovative Area "Artificial Intelligence and
Brain Science" 19H04980
The University of Tokyo
https://ror.org/057zh3y96
Center for Integrative Science of Human Behavior (CiSHuB)
Japan Society for the Promotion of Science
https://ror.org/00hhkn466
Grant-in-Aid for Scientific Research
Japan Society for the Promotion of Science
https://ror.org/00hhkn466
Grants-in-Aid for Innovative Area 18H04881
Japan Society for the Promotion of Science
https://ror.org/00hhkn466
Grant-in-Aid for challenging Exploratory Research 18K19323
Japan Society for the Promotion of Science
https://ror.org/00hhkn466
Grant-in-Aid for Scientific Research 19K06952
The Salt Science Foundation*
1728
Japan Society for the Promotion of Science
https://ror.org/00hhkn466
19K1628
The Salt Science Foundation*
2043
The Salt Science Foundation
1728
2021-01-22T00:00:00Z
2021-01-22T00:00:00Z
en
https://doi.org/10.7554/eLife.55701
206355117 bytes
6
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The ability of animals to process dynamic sensory information facilitates
foraging in an ever changing environment. However, molecular and neural
mechanisms underlying such ability remain elusive. The ClC anion
channels/transporters play a pivotal role in cellular ion homeostasis
across all phyla. Here we find a ClC chloride channel is involved in salt
concentration chemotaxis of C. elegans. Genetic screening identified two
altered-function mutations of clh-1 that disrupt experience-dependent salt
chemotaxis. Using genetically encoded fluorescent sensors, we demonstrate
that CLH-1 contributes to regulation of intracellular anion and calcium
dynamics of salt-sensing neuron, ASER. The mutant CLH-1 reduced
responsiveness of ASER to salt stimuli in terms of both temporal
resolution and intensity, which disrupted navigation strategies for
approaching preferred salt concentrations. Furthermore, other ClC genes
appeared to act redundantly in salt chemotaxis. These findings provide
insights into the regulatory mechanism of neuronal responsivity by ClCs
that contribute to modulation of navigation behavior.
Each dataset are collected by appropriate methods. For detailed
methods related to these data, please refer to "Materials and
methods" of the article "Roles of the ClC chloride channel CLH-1
in food-associated salt chemotaxis behavior of C. elegans".
For statistic analysis data, please refer to "Supplementary File 5
statistics" of the supplementary file of the article.