10.5061/DRYAD.4T9T656
Qureshi, Alima
Imperial College London
Aldersley, Andrew
Imperial College London
Hollis, Brian
École Polytechnique Fédérale de Lausanne
Ponlawat, Alongkot
Armed Forces Research Institute of Medical Science
Cator, Lauren J.
Imperial College London
Data from: Male competition and the evolution of mating and life history
traits in experimental populations of Aedes aegypti
Dryad
dataset
2019
Aedes aegypti
male mosquito behaviour
reproductive control
2019-05-21T22:27:23Z
2019-05-21T22:27:23Z
en
https://doi.org/10.1098/rspb.2019.0591
182121 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Aedes aegypti is an important disease vector and a major target of
reproductive control efforts. We manipulated the opportunity for sexual
selection in populations of Ae. aegypti by controlling the number of males
competing for a single female. Populations exposed to higher levels of
male competition rapidly evolved higher male competitive mating success
relative to populations evolved in the absence of competition, with an
evolutionary response visible after only five generations. We also
detected correlated evolution in other important mating and life history
traits, such as acoustic signalling, fecundity and body size. Our results
indicate that there is ample segregating variation for determinants of
male mating competitiveness in wild populations and that increased male
mating success trades-off with other important life history traits. The
mating conditions imposed on laboratory-reared mosquitoes are likely a
significant determinant of male mating success in populations destined for
release.
Male Mating CompetitivenessResults of mating competition experiments. For
each individual mating trial the block and replicate are identified. The
competitor treatment (HMC and NMC) is used to identify which selective
treatment was in competition with the UA line. A “1” score in the
Competitor Mate Column indicates that either the NMC or HMC males was
successful at forming a copula. A “0” score here indicates that a UA male
was successful. We additionally, have included which colour dust was used
to mark each treatment and the winglength of each male. We used a
generalized linear mixed effect model (GLMM) with a binomial distribution
to test for the fixed effects of treatment (HMC/NMC), male dust colour
(pink/yellow) and male wing length on the whether a population male formed
a copula with the U female in competition with U males. We incorporated
replicate pair, population, and block into our models as random effects.
We used a similar approach to test for the effect of treatment on whether
sperm was transferred. A GLMM with a binomial distribution was used to
test for the fixed effect of treatment (HMC/NMC) on whether a mating
resulted in successful sperm transfer. Replicate pair, population and
block were again incorporated as random
effects.Mating_in_Competition.csvMating and Acoustic Signalling in
Isolated PairsThis dataset contains the acoustic and behavioural outcomes
between one on one interactions between HMC and NMC males presented with a
tethered UA female. We used a GLMM with a binomial distribution to test
for the fixed effect of treatment (HMC/NMC) on the whether harmonic
convergence was detected during an isolated mating attempt (Yes/No) with a
U female. In a separate model, we tested for the effect of convergence,
treatment, and their interaction on whether a pair formed a copula
(Yes/No). We incorporated replicate pair, population and block into our
models as random effects.One_on_OneMating.csvFemale Mating BehaviourFor
each focal female we present the data for the timing and outcome of each
mating attempt, the timing and duration copula formation, sperm transfer,
and winglength. The effect of mating regime and replicate on female mating
behaviours (attempt and copula latencies, total attempt durations, total
attempt number, copula duration) were assessed using linear mixed models
(LMM). Female treatment was incorporated as a fixed effect and replicate
as a random effect. We used a GLMM with a binomial response variable and
logit link function to assess the effect of female mating regime as a
fixed effect and replicate pair and population as random effects on the
probability of copula formation and sperm transfer to females in these
assay.Female_Behavior.csvFecundity DataData on number of eggs laid by each
female, winglength, Female ID, Replicate, and Mating Regime. A GLMM was
used to assess the effect of female treatment and wing length as fixed
effects, and replicate pair and population as random effects, on
fecundity. We made comparisons between mating regimes using a Sequential
Bonferroni post-hoc test.Female_Eggs.csvImmature Survival Proportion and
Sex RatioData on the proportion of first instar larvae which survived to
adulthood, the proportion of first instars that survived to pupation, and
the proportion of pupae that survived to emergence, the total number of
males and females emerging for each replicate tray for the Test Rearing
(selection) We determined the effect of treatment on the proportion of
first instar larvae that eventually emerged as adults using a GLMM with
treatment as a fixed effect and replicate pair, population and
experimental block as random effects. We used a GLMM to test for the
effect of treatment on the total proportion of emerging adults which were
female and incorporated replicate pair, population and block as random
effects.Test_ImmatureSurvival_SexRatio.csvDaily Emergence DataData on
individual emergence times for Test rearing . The pattern of emergence
over time was compared between mating regimes using a Mixed Effects Cox
Regression to test for the effect using the ‘Survival’
package.TestLarval_Development.csvWinglengthsIndividual female and male
winglengths. The winglengths of females and males were compared using a
LMM with treatment as a fixed effect and replicate pair, population and
block as random effects.Test_Wings.csv