10.5061/DRYAD.4NT8M
Commandatore, Francesco
Sassera, Davide
University of Milan
Montagna, Matteo
University of Milan
Kumar, Sujai
University of Edinburgh
Koutsovoulos, Georgios
University of Edinburgh
Thomas, Graham
University of Edinburgh
Repton, Charlotte
University of Edinburgh
Babayan, Simon A.
Centre for Immunity, Infection and Evolution
Gray, Nick
Centre for Immunity, Infection and Evolution
Cordaux, Richard
French National Centre for Scientific Research
Darby, Alistair
University of Liverpool
Makepeace, Benjamin
University of Liverpool
Blaxter, Mark
University of Edinburgh
Data from: Phylogenomics and analysis of shared genes suggest a single
transition to mutualism in Wolbachia of nematodes
Dryad
dataset
2013
Litomosoides sigmodontis
Wolbachia
Symbiosis
Wolbachia pipientis
Dirofilaria immitis
2013-09-06T16:40:02Z
2013-09-06T16:40:02Z
en
https://doi.org/10.1093/gbe/evt125
798616 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Wolbachia, endosymbiotic bacteria of the order Rickettsiales, are
widespread in arthropods but also present in nematodes. In arthropods, A
and B supergroup Wolbachia are generally associated with distortion of
host reproduction. In filarial nematodes, including some human parasites,
multiple lines of experimental evidence indicate that C and D supergroup
Wolbachia are essential for the survival of the host, and here the
symbiotic relationship is considered mutualistic. The origin of this
mutualistic endosymbiosis is of interest for both basic and applied
reasons: How does a parasite become a mutualist? Could intervention in the
mutualism aid in treatment of human disease? Correct rooting and
high-quality resolution of Wolbachia relationships are required to resolve
this question. However, because of the large genetic distance between
Wolbachia and the nearest outgroups, and the limited number of genomes so
far available for large-scale analyses, current phylogenies do not provide
robust answers. We therefore sequenced the genome of the D supergroup
Wolbachia endosymbiont of Litomosoides sigmodontis, revisited the
selection of loci for phylogenomic analyses, and performed a phylogenomic
analysis including available complete genomes (from isolates in
supergroups A, B, C and D). Using ninety orthologous genes with reliable
phylogenetic signals, we obtained a robust phylogenetic reconstruction,
including a highly supported root to the Wolbachia phylogeny between an
(A+B) clade and a (C+D) clade. While we currently lack data from several
Wolbachia supergroups, notably F, our analysis supports a model wherein
the putatively mutualist endosymbiotic relationship between Wolbachia and
nematodes originated from a single transition event.
For_DRYADThe file is a zip archive of four files:
Wolbachia_phylogenomic_90genes_concatenate.faa (the catenated aminoacid
alignemtn of 90 genes), Wolbachia_phylogenomic_90genes_concatenate.ffn
(the catenated nucleic acid alignemtn of 90 genes),
Wolbachia_phylogenomic_OrthoMCL_cluster_aminoacidic_multifastas.zip (a zip
file of the amino acid/protein sequences of clusters generated from the
Wolbachia genomes using orthoMCL) and
Wolbachia_phylogenomic_OrthoMCL_cluster_nucleotidic_multifastas.zip (a zip
file of the nucleic acid sequences of clusters generated from the
Wolbachia genomes using orthoMCL)